HPLC-ELSD和HPLC-UV技术测定三七浸膏中人参.pdfVIP

HPLC-ELSD 和 HPLC-UV 技术测定三七浸膏中人参 皂苷 Rg1 含量 王素兰，洪灵鸿 （浙江大学药学院，杭州 310058 ） 【摘要】 目的：用HPLC-ELSD和HPLC-UV测定三七浸膏中人参皂苷Rg1 的含量，并比较 两种检测方法的优劣。 方法：HPLC-UV法，采用Agilent C18色谱柱，流动相：乙腈-水（20 ： 80 ），检测波长：203nm ，流速：1.0mL/min，进样量：20µL，柱温为室温；HPLC-ELSD 法，采用Tigerkin C18 （4.6mm ×150mm，5µm）柱，流动相：乙腈-水（39.5：60.5）， 流速：1.0 mL/min，ELSD检测器漂移管温度：45℃，载气压力为0.3MPa 。 结果：HPLC-UV 法测得三七浸膏中Rg1 的百分含量为28.5% ；HPLC-ELSD法测得三七浸膏中Rg1 的百分含 量为 33.2% 。UV检测器基线受流动相梯度改变的影响，在分析过程中基线的漂移较大，可 影响定量分析结果；ELSD检测器可排除溶剂的干扰，基线噪音小，峰形尖锐，改变流动相 比例时，基线不受影响。 结论：两法都较准确、可靠。ELSD检测器更适于皂苷类物质的 分析。 关键字：高效液相色谱；蒸发光散射检测技术；紫外检测技术；三七；人参皂苷 Rg1；含 量测定 Determination of Ginsenoside Rg1 in Panax Notoginseng by HPLC-ELSD and HPLC-UV Wang Su-lan , Hong Ling-hong (The College of Pharmaceutical Sciences in Zhejiang University , Hangzhou 310058) [Abstract] Objective: Establish methods for determination of ginsenoside Rg1 in Panax notoginseng by HPLC-UV and HPLC-ELSD, and compare the two methods in merits and demerits . Method: The separation by HPLC-UV was performed on Agilent C18 column with acetonitrile - water (20:80) as mobile phase, detection wavelength at 203nm,1.0mL/min of flow rate and 20μL of injection volume.The separation by HPLC-ELSD was using Tigerkin C18 (4.6mm × 150mm, 5μm) column , acetonitrile - water (39.5:60.5) as mobile phase and 1.0 mL / min of flow rate . ELSD detector drift tube temperature was 45 ℃.Carrier gas pressure was 0.3MPa. Results: HPLC-UV measured Rg1 of Panax notoginseng extract the percentage content of 28.5%; HPLC-ELSD measured Rg1 of Panax notoginseng extract content of 33.2% . Conclusion: The methods are both accurate and reliable. ELSD detector is more suitable for the analysis of saponin. [Key words] HPLC; ELSD; UV; Notoginseng; ginsenoside