利用PMSG和LRH-A3诱导间情期秋田犬发情效果的研究精要.doc

利用PMSG和LRH-A3诱导间情期秋田犬发情效果的研究 摘要 为了探索提高母犬利用年限和终身产仔数。以不同剂量和给药次数的PMSG与LRH-A3组合的方法，对乏情期母犬进行外源激素诱导发情。第一组为皮下注射PMSG 1500IU∕条，仅注射一次；第二组为皮下注射PMSG 500IU∕条，少量多次，总剂量1500IU。当两组中的母犬综合征状显示最佳配种时同时注射一次LRH-A3 25ug∕条；第三组为空白对照组不注射任何药物，为母犬正常状态。结果，第二组采用少量多次皮下注射PMSG（500IU）进行诱导发情的发情率为37.5%，比第一组，一次注射PMSG(1500IU)的发情率75%，高37.5%（P0.05）。而第一组的受胎率为33.33%，第二组的受胎率是50%，两者相差16.67%（P0.05）。试验证明，在外源性激素PMSG诱导母犬发情排卵时，在配种的同时应用LRH-A3可以促进卵泡发育和排卵，增加可用胚胎数和提高受胎率。 关键词：孕马血清,促性腺激素释放激素，秋田犬，诱导发情 Utilization of estrus Estrus Akita between PMSG and LRH-A3 induced Abstract In order to explore the use of age and improve lifelong bitches litter size. In PMSG and methods LRH-A3 combinations of different dosage and administration times of anestrus bitches exogenous hormones induce estrus. The first group was injected subcutaneously PMSG 1500IU / bar, injected only once; the second group was injected subcutaneously PMSG 500IU / bar, a few times, a total dose of 1500IU. When two of the best breeding bitch syndrome-like display simultaneously injected once LRH-A3 25ug / bar; the third group was the control group were not injected any drug for bitches normal state. As a result, the second group received a small amount of multiple subcutaneous PMSG (500IU) for induction of estrus estrus was 37.5%, compared with the first group, the first injection of PMSG (1500IU) 75% of estrus, high 37.5% (P 0.05) . The first group pregnancy rate was 33.33%, the second group pregnancy rate is 50%, a difference of 16.67% (P 0.05). Tests show that exogenous hormone PMSG inducing ovulation during estrus bitches in breeding at the same time apply LRH-A3 can promote follicular development and ovulation, increase the number of available embryos and increase conception rate. Keywords: pregnant mare serum gonadotropin-releasing hormone, Akitas, induce estrus 目 录 前 言 1 1 材料与方法 2 1.1 材料 2 1.1.1 试验时间和地点 2 1.1.2 试验动物 2 1.1.3 试验仪器与药品 2 1.2 方法 2 1.2.1 试验犬的饲养管理 2 1.2.2 激素处理方案及妊娠确定条件 2 1.2.3 外部观察法、试情法及最佳配种时间确定 3 1.2.4 阴道涂片镜检及最佳配种时间确定 3 2 结果与分析 4 2.1 注射一次PMS