kiyik河胡杨内生细菌多样性分析及一株潜在新种的多相分类学鉴定大学学位论文.docVIP

硕士研究生学位论文 论文题目（中文）论文题目（外文）：Diversity of Endophytic Bacteria from the Populus euphratica 研 究 生 姓 名：学科专业：研 究 方 向：导师 姓名 职称：艾尔肯热合曼 教授 论文答辩日期 年月 日 学位授予日期 年 月 日 Magisterial Thesis Diversity of Endophytic Bacteria from the Populus euphratica in kiyik ancient river and taxonomic identification of a novel bacterium Master Degree Candidate: Buayshem Hamood Mentor: Prof. Erkin Rahman College of Life Science and Technology, Xinjiang University摘 要本研究对343年的古河道胡杨林胡杨茎秆液进行可培养细菌的多样性分析表型和16S rRNA基因序列系统发育分析结果，株菌归类为厚壁菌门Firmicutes）、放线菌门（Actinobacteria）、α-变形菌纲（Alphaproteobacteria）、γ-变形菌纲（Gammaproteobacteria）Bacteroidetes）等5个类群。Pseudomonas)是占优势属，占分离菌株的43.75% (35/80)。 在80株细菌当中发现与Pseudomonas xinjiangensis、Pseudomonas sabulinigri、Pseudomonas azotifigens、Pseudomonas pelagia、Rhizobium rosettiformans、Microbacterium hydrocarbonoxydans、Microbacterium sedminis 、Halomonas sulfidaeris、Halomonas meridiana、Halomonas desiderata、Nesterenkonia aethiopica、Nesterenkonia suensis 、Nocardioides alkalitolerans、Planococcus maritimus、Pontibacter salisaro、Planomicrobium koreense和 Pantoea brenneri等模式菌株有着低于98.7%的RNA基因序列相似性好氧生长温度是-45℃（最适℃），pH范围是6.0-9.0（最适pH 7.0），NaCl浓度范围是0-3%（w/v）（最适是0-1%）Pseudomonas xinjiangensis S3-3T有97.24%的16S rRNA基因序列基因组DNA的G+Cmol%含量是%。16:0、Summed Feature 8、C17:0 cyclo、C19:0 cyclo w8c、C12:0、Summed Feature 3、C12:0 3OH和C10:0 3OH。基于表型特征，化学分类特征和遗传学特征分析结果，提议将菌株KBL-4-9归类为假单胞菌属的一个新物种，并命名为Pseudomonas Tograkense 。 关键词：Kiyik古河道；胡杨；内生细菌；多样性；16S rRNA； 多相分类学 Abstract The aim of this study was to identify the culturable endophytic bacteria isolated from storage liquid of six Populus Euphratica stands at the ancient Kiyik River which dried up 343years before. Through conventional dilution-plating method, we isolated 80 pure cultivable strains. Among them, 25 strains were isolated on LB plates, 21 were isolated on NB plates, 16 were isolated on KB plates and 18 strains were isolated on MA plates. Gram staining and simple staining test results of the isolates shows tha