大螟不同地理种群的AFLP分析-应用昆虫学报.doc

大螟不同地理种群的AFLP遗传分析 张志春, 罗光华, 张谷丰, 韩光杰, 刘宝生, 方继朝 （江苏省农业科学院植保所） 摘 要：为了明确我国大螟Sesamia inferens Walker不同地理种群的遗传多样性水平，利用AFLP方法对我国长江流域和南方稻区大螟的10个地理种群进行了遗传多样性分析，结果表明不同地理种群间具有丰富的遗传多样性，利用27对引物共扩增到1055条条带，其中837条条带具有遗传多态性，多态性比例为78.75%；种群间分化系数（Gst）为0.7132；Nei’s基因多样性（h）范围在0.2969～0.4547之间，平均为0.3752；Shannon信息指数介于0.2936～0.5043之间，平均为0.3888；10个种群间遗传一致度较高，均大于0.63。遗传距离聚类分析，个群可分为类德阳，，为一类AFLP；遗传多态性 Genetic diversity of different geographical populations of Sesamia inferens Walker by AFLP ZHANG Zhi-Chun, LUO Guang-hua, ZHANG Gu-Feng, HAN Guang-jie, LIU Bao-Sheng, FANG Ji-chao (Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; * Corresponding author, E-mail: fangjc@jaas.ac.cn) Abstract: To study the mechanism of the genetic diversity of different Sesamia inferens Walker populations, ten characteristic geographical populations from ten provinces of China were selected. With 37 pairs of AFLP selective primers, 1055 AFLP loci were recorded, of which 78.75% were polymorphic. Coefficient of gene differentiation (Gst) between populations was 0.7132, Nei’s gene diversity index (h) from 0.2969 to 0.4547 and the average was 0.3752, Shannon’s index from 0.2936 to 0.5043 and the average was 0.3888. There was high genetic identity between the 10 populations with the value greater than 0.63. NJ cluster analysis showed the ten populations are grouped into four major clades based on polymorphism of AFLP markers, of which DY, LH and ZJ populations belong to three different clades, and the other seven populations belong to one clade. The populations within each of the clades are consistently subject to similar geographic climate and near distance. However, the isolated population with longer genetic distance to all other tested populations was probably clustered into a random clade. So the sampled populations for genetic polymorphism clustering should be as many as possible and dispersed. Key words: Sesamia inferens; geographic population; AFLP; genetic diversit