Mfold web server for nucleic acid folding and hybridization prediction-英文文献.pdfVIP

3406–3415 Nucleic Acids Research, 2003, Vol. 31, No. 13 DOI: 10.1093/nar/gkg595 Mfold web server for nucleic acid folding and hybridization prediction Michael Zuker* Department of Mathematical Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, US Received February 14, 2003; Revised and Accepted April 7, 2003 ABSTRACT (Doug H. Turner, Department of Chemistry, University of Rochester, Rochester, NY) were used (17). The abbreviated name ,‘mfold web server’ ,describes The ‘mfold’ software for RNA folding was developed in the a number of closely related software applications late 1980s (18). The ‘m’ simply refers to ‘multiple’. The core available on the World Wide Web (WWW) for the algorithm predicts a minimum free energy, DG, as well as prediction of the secondary structure of single minimum free energies for foldings that must contain any stranded nucleic acids. The objective of this web particular base pair. Any base pair, r 7r , between the ith i j server is to provide easy access to RNA and DNA nucleotide and the j th nucleotide that is contained in a folding folding and hybridization software to the scientific no more than ddG from the minimum, is plotted in a triangular plot called the ‘energy dot plot’. The base pair r 7r is plotted community at large. By making use of unive