白菜脱水应答转录因子BpDREB1基因的克隆及功能研究.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2013, 39(2): 230237 http :///zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2013.00230 白菜脱水应答转录因子BpDREB1 基因的克隆及功能研究 刘晓颖 陈丽媛 张竞秋 李嘉玮 高 越 王振英* / , 300387 : DREB1/CBF , DREB BpDREB1 (GenBank EF219470) 647 bp, 213 , 23 kD, 5.11, 94% , BpDREB1 DREB A1 , BpDREB1 , , BpDREB1 , BpDREB1 , : ; BpDREB1 ; ; Isolation and Functional Analysis of a New DREB Transcription Factor (BpDREB1) from Brassica pekinensis * LIU Xiao-Ying, CHEN Li-Yuan, ZHANG Jing-Qiu, LI Jia-Wei, GAO Yue, and WANG Zhen-Ying Tianjin Key Laboratory of Cytogenetical and Molecular Regulation / College of Life Sciences, Tianjin Normal University, Tianjin 300387, China Abstract: DREB1/CBF transcription factors play an important role in plant stress tolerance, and one of important significance to gain stress-tolerant crops by transgenic technologies. In this study, a DREB-like gene, named BpDREB1 (GenBank accession No. EF219470), was cloned from Chinese cabbage. The BpDREB1 cDNA was 647 bp in length, and encoded protein of 213 amino acids with an predicted molecular weight of 23 kD and a isoelectric point of 5.11, and shared 94% similarity with other DREB transcription factor from Chinese cabbage. On the basis of multiple sequence alignment and phylogenetic analysis, BpDREB1 was classified in A-1 group of the DREB family. The expression patterns analysis indicated that BpDREB1 was strongly up-regulated at low temperature, also responded to dehydration. However, the expression of BpDREB 1 was not affected by high salinity. The expression