患爆发性败血症斑点叉尾触病原的分离与鉴定.pdfVIP

安徽农业大学学报，2014，41(1)：24—29 ． JournalofAnhuiAgriculturalUniversity 网络出版时间：2013．12．17 16：10：38 U【砒 http：／w／ww．cnki．net／kcms／detail／34．1162．S1610．003．html 患爆发性败血症斑点叉尾触病原的分离与鉴定 陈红莲，王永杰，陈 宇，潘庭双，吴明林，凌 俊 (安徽省农业科学院水产研究所，合肥 230031) 摘 要：从患爆发性败血症斑点叉尾鲴肝、脾和肾分离到大量形态和色泽一致的细菌。随机挑选 5株细菌注射 健康斑点叉尾鲴，感染鱼 72h内全部死亡，且患病症状与 自然发病症状一致，确认分离株为斑点叉尾鲴败血症的 致病菌。依据其菌落形态、生理生化特征，结合其 16SrRNA和gyrB基因序列分析，鉴定5个分离株为蜡样芽孢 杆菌。用 20种抗微生物药敏纸片对分离株进行药敏实验，发现 5株菌均对 7种喹诺酮类药物、5种氨基糖苷类药 物，麦迪霉素，克林霉素和新生霉素敏感，而对 2种头孢菌素类药物和复方新诺明有耐药性。 关键词：斑点叉尾鲴；败血症；蜡样芽孢杆菌；16SrRNA；gyrB 中图分类号：$941．42 文献标识码：A 文章编号：1672—352X(2014)01—0024—06 Isolation，identificationofpathogenicbacteriafrom Ictaluruspunctatus withfulminatesepticemia CHENHonglian，WANGYongjie，CHENYu，PANTingshuang，WUMinglin，LINGJun (FisheriesResearchInstitute，AnhuiAcademyofAgriculturalSciences，Hefei230031) Abstract：Largecolonieswithsimilarmorphologyandcolorwereobtainedfrom liver,spleenandkidneyof channelcatfish，／ctaluruspunctatuswithfulminatesepticemia．Theexperimentwascarriedoutwithhealthyiuve— nilechannelcatfishbymeansofiniectionwithlive5isolaresrandomlyselected．A11individualsofthefishin— jecteddiedwithin72h．Thesymptomwassimilartothoseofthenaturallyinfectedfish．Therefore，theisolates weresuggestedtobethepathogenicbacteriam strainof／ctaluruspunctatuswithfulminatesepticemia．Theseiso． 1ateswerefurtheridentifiedasBacilluscereusbyphenotypiccharacters．analysisoftheirl6SrI AandgyrBse— quences．Antimicrobialsensitivitypatternsoffivestrainswereevaluatedagainst20antimicrobialdrugs．There— suitshowedthattheywereallsensitivetosevenquinolones，fiveaminoglycosides，midecamycin，clindamycin， novobiocin，andresistanttotwocephalosporinsandcompoundsulfamethoxazole． Keywords：channelcatfish(1ctaluruspunctatus)；septicemia；Bacilluscereus；16SrRNA； rB 斑点叉尾鲴 (1ctaluInspunctatus)原