地塞米松通过PI3KAkt途径增强L02细胞P-糖蛋白的-第三军医大学学报.docVIP

DOI：10.16016/j.1000-5404.201501030 地塞米松增强L02细胞P-糖蛋白的表达作用机制的初步研究 陈 晴，谢桂娟，赵 博，张辉艳，张绪清 （400038 重庆，第三军医大学西南医院全军感染病研究所，感染病研究重庆市重点实验室 [中图法分类号] R575.3；R34；Q291 [文献标志码] A Dexamethasone increases P-glycoprotein expression in L02 cells via activation of PI3K/Akt signaling Chen Qing1, Xie Guijuan1, Zhao Bo1, Zhang Huiyan1, Zhang Xuqing（Institute of Infectious Diseases, Chongqing Key Laboratory for Infectious Diseases Research, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China .） ［Abstract］ Objective The aims of the present study were to investigate the effects of dexamethasone (Dex) on P-glycoprotein (P-gp) expression in human normal liver cell line L02 cells and the role of phosphatidylinositol 3 kinase (PI3K) /protein kinase B (Akt) pathway in the regulation of P-gp expression. Methods The levels of P-gp expression were detected among untreated, Dex alone treated, PI3K/Akt inhibitor LY294002 alone treated, and both Dex and LY294002 treated L02 cells using western blot assay. The levels of Akt expression were also detected in untreated and Dex treated L02 cells using western blot assay. Otherwise, we also investigated the relationship of P-gp and Akt expression in L02 cells. Results The levels of P-gp and Akt expression were significantly increased for 60.8% and 25.0% in L02 cells treated with 10μM Dex for 24h, compared with those in untreated L02 cells (P=0.0267, 0.0358). The levels of P-gp expression were positive correlated with the levels of Akt expression in L02 cells (P＜0.01). The levels of P-gp expression were significantly decreased for 33.8% in LY294002 treated L02 cells, compared with that in untreated L02 cells (P=0.0184). The levels of P-gp expression in Dex alone treated L02 cells were 163.5±18.4% of untreated cells, which were significantly higher than 130.1±22.0% of untreated cells in L02 cells treated by combination of Dex and LY294002（P=0.0313）. Conclusion Dex may increase the levels of