Na2CO3和NaCl对大豆种子萌发胁迫效应的比较研究.pdfVIP

研究报告 寇 贺等：N％CO，和NaC1对大豆种子萌发胁迫效应的比较研究 Naz CO3和NaC1对大豆种子萌发胁迫效应的比较研究 寇 贺， 曹敏建，那桂秋 (1．沈阳农业大学，辽宁沈阳110161；2．辽宁省农科院，沈阳110161) 摘要：大豆种子在Na 浓度依次为1O、2O、4O、8O、100 mmol／L的NaC1和N CO 溶液中进行胁迫处理，然后调查相对发 芽率、计算活力指数，测定下胚轴长度和直径、胚根长、侧根数，有机酸、脯氨酸、可溶性糖和MDA的含量及SOD、POD的 活性。结果表明，N％CO3和NaC1对大豆种子萌发胁迫效应不一样。相比之下，在Na2c03胁迫下，低Na 浓度处理对大 豆种子相对发芽率和活力指数、下胚轴和胚根有抑制作用，并且对各指标的抑制程度均大于NaC1胁迫，同时随胁迫程度 的增大，抑制作用越明显。在适应N％CO，胁迫过程中耗能过多，活性氧清除系统失衡，膜系统受到严重损害，说明 N CO 胁迫对大豆萌发的伤害作用远大于NaC1。 关键词： 大豆；萌发；N％CO ；NaC1；胁迫 中图分类号：$565、1 文献标志码： A 文章编号： 1001-4705(2007)12-0027-05 Comparative Studies on Reaction at Germination Stage of Soybean under Na2 CO3 and NaC1 Stresses KOU He，Cao Min-jian，NA GlIi-qiu (Shenyartg Agriculture University，Shenyang Liaoning 1 10161，China) Abstract：Seed of soybean were treated with different concentrations of NaC1 and Na，CO1(Na concentrations isl0，20，40，80 and 100 mmol／L．Then the changes in relative germination percentage、vigor index，hypocotyls length and diameter、radicle length、lateral root numbers．the activity of SOD and POD and the contents of MDA，organic acids、proline、soluble sugar were determinated．The results showed that the changes in soybean at the germination stage under Na2 CO3 stress were different from under the NaC1 stress．Although the growth of soybean seeds could be inhibited by both Na2 CO3 and NaC1 stresses and the inhibition was aggravated with the increasing salinity，for the effect of high pH value(Na concentration under the same leve1)，the extent of in— hibition resulted from Na2 CO3 stress was greater than that from NaC1 stress．Compared with NaC1 treatment，un— der Na2 CO3 stress：(1)relative germination percentage、vigor index；(2)hypocotyls and radicle length、lateral root numbers wer