Polyphen2和SIFT教程.pptVIP

用Polyphen2和SIFT进行突变/SNP功能预测;三个网址;数据准备;获取蛋白质序列或ID;查询结果;点击所需要的蛋白质ID链接，在出现的页面中可以详细查看DAX-1的信息，再次核对是否正确，注意右上角的几列标签，如图;Polyphen2应用;运行画面;结果;练习;SIFT;SIFT预测AR S578N功能变化;sp|P10275|ANDR_HUMAN Androgen receptor OS=Homo sapiens GN=AR PE=1 SV=2 MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQ QQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSA LECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLK DILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKA VSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKS TEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSR DYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGP GSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPY GYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLE TARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRND CTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLT VSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAK ALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRM YSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDR IIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIIS VQVPKILSGKVKPIYFHTQ ;将刚才编辑好的数据填入这个框中（之前介绍过这个数据输入框）;OK！;结果会有一堆英文，看了头痛，直接找到Scaled Probabilities for Entire Protein和Predictions of substitutions entered两处链接，分别点击进去。 Scaled Probabilities for Entire Protein给出了所提交氨基酸每个位点发生突变后的计算分数，只要分数小于0.05就认为可能影响到蛋白质功能 而Predictions of substitutions entered直接给出预测答案;Scaled Probabilities for Entire Protein结果图示;Predictions of substitutions entered结果;就简单介绍到这，谢谢大家耐心看完 有什么问题的话可以发邮件给我交流 拜拜，我睡觉了…… 下面还有哦;满 月 咯;我在等爸爸回家哦;