管理资料低氧对脂肪间充质干细胞成软骨分化的诱导作用.pdf

Cell Tissue Res DOI 10.1007/s00441-013-1732-5 REGULAR ARTICLE Hypoxia enhances chondrogenic differentiation of human adipose tissue-derived stromal cells in scaffold-free and scaffold systems Samir Munir Casper Bindzus Foldager Martin Lind Vladimir Zachar Kjeld Søballe Thomas Gadegaard Koch Received: 7 June 2012 /Accepted: 5 September 2013 # Springer-Verlag Berlin Heidelberg 2013 Abstract Human adipose-derived stromal cells (hASCs) pos- with chemical induction using TGF- β3. hASCs from four sess the potential for chondrogenic differentiation. Recent stud- human donors were cultured both in a micromass pellet system ies imply that this differentiation process may be enhanced by and in 3D collagen I/III scaffolds in either 5 or 21 % O2. culturing the cells in low oxygen tension in combination with Chondrogenesis was evaluated by quantitative gene expression three-dimensional (3D) scaffolds. We report the evaluation of analysis of aggrecan, SOX9, collagen I, II and X and histolog- the chondrogenic potential of hASC pellets in 5 and 21 % O2 ical evaluation with HE and toluidine blue staining. Induced and as cell-scaffold constructs using a collagen I/III scaffold pellets cultured in 5 % O2 showed increased peripheral cellu- larity and matrix deposition compared with 21 % O2. Induced pellets cultured in 5 % O2 had increased control-adjusted gene S. Munir : C. B. Foldager :K. Søballe expression of aggrecan, SOX9 and collagen I and decreased Orthopaedic Research Laboratory, Institute for Clinical Medicine, collagen X compared with 21 % O2 cultures. Induced pellets Aarhus University Hospital, Noerrebrogade 44 Bldg 1a, had higher gene