基因工程在石油污染应用.pptVIP

基因工程在石油污染治理中的应用Application of genetic engineering in petroleum pollution control ;目录contents;一、石油污染;1.Petroleum pullution;;二、基因工程;2.Genetic engineering;概括起来，基因工程的操作过程一般分4个步骤。 1、获得目的基因； 2、将目的基因与载体连接形成重组DNA； 3、将重组DNA导入受体细胞； 4、筛选出能表达目的基因的受体细胞;Generally, the operation process of genetic engineering is generally divided into 4 steps. 1, to obtain the target gene; 2, the recombinant DNA was formed by connecting the target gene and the vector; 3, the recombinant DNA into the receptor cells; 4, the receptor cells that can express the target gene were screened out.;否溪剔崭蚕寐旷烯绊虞非籍蠕嗽蕉麓振邱怖嫉虞醚船烈容染庇娟呸摸帜灶基因工程在石油污染应用基因工程在石油污染应用;三、基因工程治理石油污染;3.Genetic engineering treatment of petroleum pollution;1975年美籍印度人查克拉搏特等科学家依据似单胞杆菌对石油中有毒成分具有很强分解力这一特性，把4种能吃浮油的假单胞杆菌的基因拼接起来，合并成一种假单胞菌种．组成了所谓的“超级菌”，它能分解各种石油烃,消除浮油的效率高、速度快，只需几小时就能除掉自然菌种需几年才能消除的原油污染。经不断改进．这种超级细菌已成功地用于实际环境治理中。; 1975 American Indians check carat stroke on the basis of the principal scientists like subsp.achromogenes has a strong decomposition of the characteristics of toxic components in the oil, the four can eat oil slick Pseudomonas genes were spliced together, merged into a Pseudomonas species. Composition of the so-called super bacteria, it can decompose various petroleum hydrocarbon and eradicate the high efficiency of the oil slick, fast speed, just a few hours will be able to get rid of natural strains for several years to eliminate oil pollution. Through continuous improvement. This super bacteria have been successfully used for actual environmental governance.;（一）石油烃降解菌种类Types of petroleum hydrocarbon degrading bacteria;（二）石油烃微生物降解的影响因素Influencing factors of microbial degradation of petroleum hydrocarbons;（三）微生物对石油烃的代谢途径和机理 ;3.3 Metabolic pathways and mechanisms of microorganisms for petroleum hydrocarbons ;（四）石油污染的微生物降解途径Microbial degradation pathway of oil pollution;2、烯烃的降解途径;3、芳香族的生物降解Biodegradation of aromatic compounds;4、环烷烃的生物降解Biodegradation of cycloalkanes;5、多环芳烃Polycyclic aromatic hydrocarbons;四、小结Sum