培养大鼠脑皮层神经元损伤后c-fos蛋白表达(国外英语资料).doc

培养大鼠脑皮层神经元损伤后c-fos蛋白表达(国外英语资料) The c-fos expression was developed after the injury of neurons in the brain cortex Update date: 2010-09-08 king of China, zhu pei fang of the state of zhu pei fang 【 abstract 】 objective to observe the role of c-fos in the secondary brain injury after the injury of neurons in rats. Methods the cortex neurons of the 10 to 12 day SD mice were damaged by glutamate and mechanical scratch. Using SP, the double marking of immunisation was carried out. As a result, two hours after glutamate, c-fos began to be expressed, and in four hours they were clearly concentrated in the nucleus. The two hours after the trauma had been shown in the direct lesion on the edge of the channel, the c-fos were expressed, and the neurons that were far away from the direct damage area were shown four hours after the trauma. The small number of neurons protruding from the distal end of the neuron, and the c-fos particle; Six hours after the injury, the neurons began to disintegrate, but the c-fos particles did not disappear. Conclusion glutamate injury can cause cultured neurons c - fos protein expression of cultured cortical neurons and glial cells after injury can release toxic substances, training after injury of neurons - c fos protein expression may be associated with apoptosis. 【 keywords 】 c-fos neurons develop glutamate secondary brain injury The Expression of c-fos After Cultured Cortical Neuron Injury in Rats WANG ke-wan, YANG Zhi -huan, WANG zheng-guo, et al. Research Institute of Surgery, Third Military Medical University, Chongqing 400042 【Abstract】　Aim　To observe the expression of c-fos protein in cultured cortical neurons subject to either glutamate or mechanical injury in rats, and to study the effect of c-fos protein on brain injury. Methods　Mixed cultured cortical neurons from SD rats were injured by either glutamate or mechanical force, then the expression of c-fos protein was studied with double-labeled immunohistochemistry. Results　Two hours