液相色谱分析实验(国外英语资料).docVIP

液相色谱分析实验(国外英语资料) Liquid chromatography experiment I. e. Understand and understand the process of high-performance liquid chromatograph. Grasp the qualitative and external quantitative method of liquid chromatography. Understand the classification of liquid chromatography and chromatographic column. Two, [experimental principle] The theory of separation of liquid chromatography: Where K is the distribution coefficient, K prime is the capacity factor, Vm. V is the volume of the moving phase and the fixed phase respectively, and the p and q are the weights in the stationary phase and the moving phase respectively. Benzene, naphthalene and biphenyl, Philippines respectively belong to the ring, polycyclic aromatic hydrocarbons and polycyclic, and the intensity of polarity and molecular weight has the difference, therefore, their flow facies in strongly polar non-polar stationary phase distribution coefficient K is different between the retention time is not the same, mixed samples and can be separated in the chromatographic system. Because of benzene, naphthalene, and benzobenzene, it is absorbed by ultraviolet wavelengths, so the uv detector is tested. In the anti-phase chromatographic system, the stationary phase is nonpolar and the flow is polar. In chemical bonds and chromatography, the ligand of a fixed phase is often the alkyl of 2-18 carbon atoms. The flow phase is usually a mixture of polar organic solvents and water. In the order of dissolute polarity the order is washed away. As the movement of the polarity increases. The retention value increases as well. Reversed-phase chromatographic separation is hydrophobic solute structure differences, on the basis of the polarity of the solute, the greater the retention value is smaller, the homologue, its retention value increases with the increase of carbon number. Solvent composition of the sample retention has a very profound effect, the size of the solute retention is decreases with the decreasing of the polarity o