膀胱果种子离体萌发与植株再生.pdf

2011,40(3): 36-40. Subtropical Plant Science 膀胱果种子离体萌发与植株再生 1,2,3 2 1,2 1,2,3 1,2,3 1,2 余道平 ，李策宏 ，谢孔平 ，张国珍 ，谷海燕 ，李世丽 (1.四川省自然资源科学研究院，四川 成都 610041；2.峨眉山生物资源实验站，四川 峨眉山 614201；3.四川省生物资 源保护与可持续利用实验室，四川 成都 610041) 摘 要：以膀胱果种子为外植体，通过对基本培养基、生长调节剂配比及移栽基质的筛选，初步建立其离体培 养再生体系。结果表明，MS 培养基是较适合膀胱果种子萌芽和幼苗生长的基本培养基；诱导下胚轴脱分化成 愈伤组织的较佳培养基配方为 MS + 2,4-D 0.5 mg/L + 6-BA 0.5 mg/L；顶芽增殖培养的最佳培养基为 MS + KT 2.0 mg/L + NAA 0.2 mg/L；芽苗生根的最佳培养基为 1/2MS + NAA 1.0 mg/L；以泥炭土作为移栽基质，成活率 达 70% ，且幼苗生长健壮。 关键词：膀胱果; 种子；离体萌发；再生 Doi: 10.3969/j.issn.1009 -7791.2011.03.010 中图分类号：Q943.1 文献标识码：A 文章编号：1009-7791(2011)03-0036-05 In Vitro Germination and Plantlet Regeneration from Seeds of Staphylea holocarpa 1,2,3 2 1,2 1,2,3 1,2,3 1,2 YU Dao-p ing , LI Ce-hong , XIE Kong-ping , ZHANG Guo-zhen , GU Hai-yan , LI Shi-li (1.Sichuan Nature Resource Institute, Chengdu 610041, Sichuan China; 2.Emei Mountain Biotic Resource Experimental Station，Emei Mountain 614201, Sichuan China; 3.Sichuan Provincial Laboratory for Biotic Resource Protection and Sustainable Utilization, Chengdu 610041, Sichuan China) Abstract ：With seeds of Staphylea holocarpa as explants, its regeneration system cultured in vitro was established preliminarily through screening basic medium, growth regulator proportion and transplantation medium. The results showed that the MS medium was one suitable basic medium for its seeds germination and seedlings growth, the medium MS + 2,4-D 0.5mg/L + 6-BA 0.5mg/L could induce the hypocotyl dedifferentiat ing into callus, and MS + KT 2.0mg/L + NAA 0.2m