电子自旋共振波谱法检测辐照中成药颗粒制剂.PDFVIP

Drug Evaluation Research 第 36 卷 第 4 期 2013 年 8 月 - 293 - 电子自旋共振波谱法检测辐照中成药颗粒制剂 赵小俊，傅俊杰，汪志平，孙志明，汪海燕 浙江大学 农业与生物技术学院 原子核农业科学研究所/农业部核农学重点开放实验室，浙江 杭州 310029 摘 要：目的 利用 60Co-γ射线辐照对电子自旋共振（ESR ）波谱特性的影响，建立简便、快捷的辐照中药颗粒制剂检测方 法。方法 2 种中成药颗粒（妇乐颗粒和抗感解毒颗粒）样品分别经剂量率为 1.0 kGy/h，剂量为 0、0.1、0.3、0.7、1、3、5、 7、9 kGy 的60Co-γ射线辐照处理，用顺磁共振波谱检测仪对样品 ESR 波谱特性进行检测，样品量为 0.05 g，每个样品 3 次 重复。结果 辐照前后 ESR 波谱峰值有明显差异，ESR 信号强度随辐照样品吸收剂量的增加而增强；样品在室温下保存 120 d，ESR 信号强度随时间虽有变化，但仍处于与对照组呈显著差异的水平。结论 利用 ESR 波谱法可简便、快捷、有效地鉴 别中药颗粒制剂是否经辐照处理。 关键词：中成药颗粒制剂；电子自旋共振（ESR ）；辐照 中图分类号：R917 R954 文献标志码：A 文章编号：1674 - 6376 (2013) 04 - 0293 - 04 DOI: 10.7501/j.issn.1674-6376.2013.04.014 Detection of irradiated granules preparation of Chinese patent medicine by ESR spectroscopy ZHAO Xiao-jun, FU Jun-jie, WANG Zhi-ping, SUN Zhi-ming, WANG Hai-yan Key Laboratory for Nuclear-Agricultural Sciences of Ministry of Agriculture, Institute of Nuclear Agricultural Sciences, College of Agriculture Biotechnology, Zhejiang University, Hangzhou 310029, China Abstract: Objective To develop a simple and convenient method to detect the granules preparation of Chinese patent medicine (GPCPM), based on the effect of ESR spectroscopy using 60Co-γ ray irradiation. Methods Two kinds of GPCPM were irradiated by 60Co-γ ray under nine doses (0, 0.1, 0.3, 0.7, 1, 3, 5, 7, and 9 kGy) at 1.0 kGy/h dose rate. The ESR spectroscopy was characterized by ESR detector with 0.05 g of sample and repeated for three times. Results It was shown that the ESR spectra were obviously different with or without irradiation. The intensity of ESR signal increased with the irradiation dose increasing, but decreased with the storage time expansion with a linear correlation. The intensity of ESR was weakened gradually during the storage at the room temperature for 120 d, though the ESR signal could be still detected. Conclusion The method of ESR spectroscopy is simpl