培养条件对青枯雷尔氏菌脂肪酸组成的影响.doc

培养条件对青枯雷尔氏菌脂肪酸的影响 朱育菁 苏明星 黄素芳 王秋红 刘 波( （福建省农业科学院农业生物资源研究所 福州 350003） 摘 要: 应用气相色谱技术测定不同温度、培养时间、pH值等培养条件下青枯雷尔氏菌（Ralstonia solanacearum）脂肪酸的结果表明：青枯雷尔氏菌强致病力菌株Rs-J.1.4-010704-01v的脂肪酸种类有1～3种，主要特征脂肪酸C16:1ω7c/C15:0 ISO 2OH（10.644 min），C16:0（10.950 min），C18:1ω7c（14.177 min）～；该菌脂肪酸的种类与含量随着培养条件的改变而发生变化，经聚类分析聚成4类。在培养温度为20和25时，表现为C16:0C16:1ω7c/C15:0 ISO 2OH，属于过渡性致病力的类群。在30～、24～ h、pH 5～9、培养基LB、NA、TTC和TSB的条件下，Rs-J.1.4-010704-01v均表现强致病菌株的脂肪酸特征，即C16:0C16:1ω7c/C15:0 ISO 2OH。但是，在培养条件分别为40和48 h～96 h时，C16:0与C16:1ω7c/C15:0 ISO 2OH差值显著地增大，由上升至；同时，随着培养时间的延长，脂肪酸种类也增加至30种。研究表明：温度和培养时间比pH值和培养基种类的影响更显著。 关键词: 青枯雷尔氏菌，脂肪酸，培养条件Effects of Cultural Condition on Fatty Acid Composition of Ralstonia solanacearum ZHU Yu-Jing SU Ming-Xing HUANG Su-Fang WANG Qiu-Hong LIU Bo* (Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China) Abstract: Fatty acids of Ralstonia solanacearum cultured under different temperatures, times, pH values and cultural media were detected by using gas chromatography (GC) method. Rs-J.1.4-010704-01v, a virulent strain of R. solanacearum isolated from ginger was chosen for the experiment. The results showed that the kind of fatty acid of Rs-J.1.4-010704-01v fluctuated from 14 to 33. The contents of its three plentiful fatty acids, C16:1ω7c/C15:0 ISO 2OH, C16:0 and C18:1ω7c (with retain times of 10.644, 10.950 and 14.177 min, respectively), also varied in a range of 55.66% to 75.69%. The diversification of the bacterium’s fatty acids at various cultural conditions was clustered into four groups by cluster analysis, according to the kinds and percentage contents of the fatty acids detected. The pathogenicity of Rs-J.1.4-010704-01v under 20℃ and 25℃ were deduced to be mid-virulent, with C16:0 less than C16:1ω7c/C15:0 ISO 2OH. The bacterium showed as a virulent strain under the other cultural conditions including 30℃-40℃, 24 h-96 h, pH 5-9 and four cultural media (LB、NA、TTCTSB), with C16:0 more than C16:1ω7c/C15: