甘草内生菌Z4菌株对灰霉病菌作用机制初探.DOC

基金项目：陕西省教育厅专项科研计划项目(11JK0643)；陕西省科技攻关计划项目(2012K01-12)；陕西省教育厅省级重点实验室科研项目(12JS121)；陕西省高水平大学建设专项资金资助项目2012SXTS06)；国家大学生创新训练计划项目(201310719008)；国家大学生创新训练计划项目201310719005)；延安大学科研基金资助项目(YD200653)。 第一作者简介：刘霞，女，1970年生，陕西绥德人，副教授，博士，主要从事农药毒理学研究。通信地址：716000 陕西延安市宝塔区杨家岭延安大学生命科学学院，E-mailliuxiayidan@126.com。 收稿日期：2014-04-14，修回日期： 甘草内生菌Z4菌株对灰霉病菌的作用机制初探 刘 霞1,2,3，马绥斌1，李 佳1 （1延安大学生命科学学院，陕西延安 716000；2陕西省区域生物资源保育与利用工程技术研究中心，陕西延安 716000；3陕西省红枣重点实验室，陕西延安 716000） 摘 要：为探究甘草内生菌Z4菌株的发酵上清液对灰霉病菌的抑菌机制，采用抑制菌丝生长速率法及抑制孢子萌发法，结合扫描电镜和透射电镜，观察Z4菌株发酵上清液处理前后灰霉病菌菌丝形态结构的变化情况。采用盆栽试验测试活性物质在活体黄瓜内的传导性。通过扫描电镜、透射电镜及光镜观察发现，Z4菌株的发酵上清液使番茄灰霉病菌菌丝扭曲变形、表面皱缩、部分菌丝断裂；细胞壁溶解破裂，细胞内液泡出现空腔；孢子萌发率低及芽管生长缓慢。盆栽黄瓜灌根和涂叶处理前后，黄瓜的病指分别由82.3降至28.05以及83.11降至30.60，防治效果分别为 63.12%和60.28%。表明Z4菌株发酵上清液对灰霉病菌菌丝细胞的细胞壁具有破坏作用，且活性物质在活体黄瓜内有一定传导性。 关键词：甘草内生菌Z4菌株；灰霉病菌；作用机制 中图分类号：S432.4+4文献标码：APreliminary Investigation of Mechanism of Action of Z4 Endophytic Strain Separated from Wild Liquorice for Botrytis cirerea Liu Xia1,2,3, Ma Suibin1, Li Jia1 (1College of Life Science, Yan’an University, Yan’an Shaanxi 716000; 2Shaanxi Engineering and Technological Research Center for Conservation and Utilization of Regional Biological, Yan’an Shaanxi 716000; 3Key Laboratory of Red Dates Shaanxi Province, Yan’an Shaanxi 716000) Abstract: The study aims to investigate the antifungal mechanism of the supernatant fermentation from Z4 strain in vitro. The action mechanism of Z4 strain was surveyed by inhibiting the mycelial growth ratio and spore germination, combining scanning electronic microscope, transmission electronic microscope and potted plant. The results indicated that the fermentation extracts exhibited antifungal activities to mycelium growth of Botrytis cinerea. The fermentation extracts could inhibit the spore germination and the bud growth of Botrytis cinerea. Also the sample had a series of marked morphological and structural alterations of the mycelium under the light microscope and electronic microscope, such as prayer beads swollen of part of mycel