期净光合速率与蒸腾速率及挥发油化学成分的比较研究.docVIP

两种化学型鱼腥草不同生育期净光合速率和蒸腾速率 及挥发油化学成分的比较研究 黄春燕，吴 卫，郑有良，陈 黎，刘 雷，刘仁建 （四川农业大学农学院雅安 625014） 目的】 研究化学型鱼腥草不同生期叶片净光合速率和蒸腾速率挥发油化学成分。方法】便携式光合仪测定自然条件下两种化学型鱼腥草苗期和花期叶片的净光合速率(Pn)和蒸腾速率(Tr)日变化。水蒸气蒸馏法分别提取挥发油，并用GC-MS法对各化合物进行鉴定，峰面积归一法计算各成分相对含量。结果】两种化学型鱼腥草Pn日变化单峰曲线，Tr日变化单峰曲线Pn和Tr。两种化学型鱼腥草叶片挥发油所含苗期普遍比花期多，而共有成分和主要成分总量低于花期M型鱼腥草叶片挥发油所含化学成分种类较D型多，而其挥发油化学成分中共有成分和主要成分总量D型低结论】峨眉蕺菜与蕺菜 鱼腥草化学型；期；净光合速率蒸腾速率；挥发性成分 Analysis on Photosynthetic Rate, Transpiration Rates and Volatile Chemical Constituents from Leaves of Various Houttuynia Chemotypes at Varied Growth Stages HUANG Chun-yan, WU Wei, ZHENG You-liang, CHEN Li, LIU Lei, LIU Ren-jian (Agronomy College, Sichuan Agricultural University, Yaan 625014) Abstract:【Objective】The study compared the net photosynthetic rate (Pn), transpiration rate (Tr) and volatile chemical constituents of two different Houttuynia leaves chemotypes at different growth stages. 【Method】The diurnal variation of Pn and Tr were determined with CIRAS-1 under natural conditions. Volatile oils were obtained with steam distillation and the chemical constituents were separated and identified by GC-MS. The relative content of each constituent was determined by area normalization. 【Results】The curve of diurnal variation of Pn tended to be a typical single peak at the seedling stage and double peak type at the flowering stage. Tr diurnal variation curves at both stages were single peak types. Both Pn and Tr values at seedling stage were much higher than that at the flowering stage in all the tested materials. Meanwhile, the numbers of volatile chemical constituents were also more plentiful at the seedling stages than at the flowering stage. The total contents of common and major volatile chemical constituent were much lower at the seedling stage than at the flowering stage. The numbers of the volatile chemical Myrcene (M) chemotype were much more plentiful than Decanal(D) chemotypes at both stages. While the total contents of common and major volatile chemical constituent of M ch