胶体金免疫层析技术快速检测沙丁胺醇残留幻灯片.pdf

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.11 胶体金免疫层析技术快速检测沙丁胺醇残留 1 2 吴巧丽 ，叶春生 （1.浙江省质量检测科学研究院，浙江杭州 310013）（2.杭州博拓生物技术有限公司，浙江杭州 311121） 摘要：建立胶体金免疫层析检测法，用于动物组织或饲料样品中沙丁胺醇残留的快速检测。采用胶体金免疫竞争法，将兔抗沙 丁胺醇多克隆抗体-胶体金标记物包被在胶体金聚酯结合垫上，并将沙丁胺醇-BSA 抗原包被在硝酸纤维素膜上作为检测线，与待测试 样中的沙丁胺醇竞争结合胶体金标记的兔抗沙丁胺醇多克隆抗体，从而判断试样中是否有沙丁胺醇存在。本文优化了检测试剂，可检 测出猪肉等组织和饲料试样中沙丁胺醇的低限浓度为2 ng/mL，与盐酸克伦特罗的交叉反应率为2.67% ，且试验在3 min 内完成，肉 眼可判断结果。本检测方法检测沙丁胺醇，灵敏度高，特异性好，可用于现场的快速检测和筛查。 关键词：沙丁胺醇；免疫层析；快速检测；胶体金；竞争法 文章篇号：1673-9078(2012)11-1595-1599 Colloidal Gold Immunochromatographic Assay for Residual Sabutamol 1 2 WU Qiao-li , YE Chun-sheng (1.Zhejiang Provincial Quality Inspection Institute of Science, Hangzhou 310013, China) (2.Hangzhou Biotest Biotech Co., Ltd, Hangzhou 311121, China) Abstract: Colloidal gold immunochromatography assay can rapidly test residual subutamol in meat and animal feed. An anti-salbutamol antibody is conjugated to colloidal gold and placed on conjugate pad. Salbutamol-BSA antigen is coated on nitrocellulose membrane as test line (T line). Colloidal gold provides color to visualize antibody-antigen binding. If there is Salbutamol in the sample, it will bind gold-antibody conjugate and prevent its binding onto the Salbutamol-BSA antigen in the test line(T line) on membrane. As result, no color will be visible on test line on membrane. Otherwise, anti-salbutamol antibody conjugated to colloidal gold will bind the Salbutamol-BSA antigen leading to red color on test line. The color of test line indicates positive or negative result. With optimized reagents, the lowest limit of detection is 2ng/ml. The cross reaction with Clenbuterol is 2.67%. Res