生长激素信号肽可诱导重组蛋白外分泌表达剖析.pdfVIP

ISSN 中国生物化学与分子生物学报 2005 年 4 月 ( ) CN 　113870Q Chinese Journal of Biochemistry and Molecular Biology 21 2 :282～286 ·研究简报 · 生长激素信号肽可诱导重组蛋白外分泌表达 张志谦 , 　李金萍 , 　胡 　颖 (北京大学临床肿瘤学院暨北京市肿瘤防治研究所 ,北京 　100034) Secretion of Recombinant Proteins in Ma mmalian Cells Directed by Growth Hormone Signal Peptide ZHAN G ZhiQian , L I JinPing , HU Ying ( Dep artment of Cell B iology , Peking University School of Oncology ,Beij ing Institute f or Cancer Research ,Beij ing 　100034 , China) Abstract 　Signal peptide capable of efficiently directing many protein secretion in mammalian cells is one of the key elements in recombinant protein production ,gene therapy and the development of DNA vaccines. In order to explore the possibility of rat growth hormone signal peptide as such an element ,a new vector based on the mammalian expression vector pcDNA3 was constructed by employing rat growth hormone ( r GH) signal peptide as leading sequence ,followed by multiple cloning sites ,the myc epitopetag and 6 ×his purification tag in the expression cassette . The vector was validated by successfully expressing and secretion of chick MMP2 C terminal PEX domain ,a potential angiogenesis inhibitor ,and tandem peptide repeats of myc epitopetag in COS 7 cells. These results suggest that rat growth hormone signal peptide is effective in the mediation of recombinant protein expression and secretion , and this vector provides a new tool for universal