DNA指纹技术在可疑精子标本等鉴定中的应用.pdfVIP

( ) 中南大学学报 医学版 　2004 ,29 (5) J Cent Sout h Univ (Med Sci) 537 ① DNA 指纹技术在可疑精子标本等鉴定中的应用 朱文兵 , 傅俊江 , 刘上峰 , 范立青 , 李麓芸 , 卢光　 ( 中南大学生殖与干细胞工程研究所 ,长沙 4 10078) [摘要] 　目的:应用 DNA 指纹技术对精子库供精者中来源可疑的精子标本进行鉴定 ,并对供精者的后代进行 亲子鉴定 。方法 :分别对本研究室所涉及的 6 名供精者的精子标本及其对应可疑精子标本各一份 , 同时志愿者精子 和其儿子外周血各一份提取 DNA ,应用 PCR 扩增 ,PA GE 胶电泳检测 DNA 指纹图。结果 :通过 DNA 指纹图分析 ,5 个可疑精子标本被确定为对应同一供精者精子标本 ,余 1 例被确认为非同一供精者精子标本来源 ;志愿者和其儿子 被确定为亲子关系 。结论 :应用 DNA 指纹图技术对精子库供精者的可疑精子标本和供精者精子及对应供精者精子 和后代外周血进行分析 ,可以对其来源进行准确的鉴定 。 [ 关键词] 　DNA 指纹图; 　精子 ; 　外周血 ; 　鉴定 [ 中图分类号] 　Q75 　　　　[文献标识码] 　A 　　　　[文章编号] (2004) Identif ication of the resource suspected sperm by D NA f ingerprinting ZHU Wenbing , FU J unj iang , L IU Shangfeng , FAN Liqing , L I L uyun , L U Guangxiu ( Rep rod uction an d S tem Cell Engi neeri ng I nstit ute , Cent ral S out h University , Changs ha 4 10078 , Chi na) Abstract : 　Objective 　To identify t he resource suspected sperm of donor in human sperm bank and apply t he p arent age testing between t he donor and his off sp ring . Methods 　We took t he 6 semen specimen of t he donor involved and correspondently suspected semen as well as t he semen of one volun teer and perip heral blood of his off sp ring . All specimens were amplified by PCR , and DNA fingerp rint was detected by PA GE elect rop horesis. Results 　By DNA fingerp rinting we discovered t hat t he 5 sus pected sperm samples came from corresponding donor s and t he ot her sample from anot her . The volun teer and his off sp ring we