shRNA SNAIL 导致的细胞表型改变.pdfVIP

Mol Cell Biochem (2014) 388:113–121 DOI 10.1007/s11010-013-1903-4 The effects of shRNA-mediated gene silencing of transcription factor SNAI1 on the biological phenotypes of breast cancer cell line MCF-7 Yan Lu • Lina Yu • Minlan Yang • Xiangshu Jin • Zhijing Liu • Xiaowei Zhang • Liping Wang • Dongjing Lin • Yuanyuan Liu • Min Wang • Chengshi Quan Received: 18 August 2013 / Accepted: 15 November 2013 / Published online: 29 November 2013 Springer Science+Business Media New York 2013 Abstract To research the effects of silencing transcrip- transition process by regulating relevant target gene tion factor SNAI1 on the in vitro biological phenotypes of E-cadherin. breast cancer cell line MCF-7, based on the gene sequence of SNAI1, we linked shRNA with the green fluorescent Keywords SNAI1 Gene silencing Biological protein-expressing eukaryotic expression vector pGCsi- phenotypes MCF-7 lencerTM U6/Neo/GFP, and transfected it into MCF-7 cells. The SNAI1 gene-silencing effect was authenticated by RT-PCR and immunofluorescence. We then examined the Introduction effect of gene silencing on the expression of epithelial and mesenchymal markers and on their biological phenotypes Ductal carcinoma of the breast is one of the most common of the target cells. Finally, we explained that SNAI1 was malignant tumors in women, and its incidence is increasing bound to E-cadherin in MCF-7 cells by ChIP. Silencing year by year [ 1, 2]. Tumor metastasis is the main reason for SNAI1 upregulated the expression of epithelial markers mortality from this disease [3]. In breast cancer, the claudin-4, claudin-7, and E-cadherin, while expression of expression level of SNAI1 (snail homolog 1), a transcription the mesenchymal marker matrix metalloprotein