应用RAPD分析技术研究生姜青枯病的土壤防治.PDF

微生物学通报 MAY 20, 2009, 36(5): 678~683 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 应用RAPD 分析技术研究生姜青 枯病的土壤防治 1* 2 陆 合 张碧波 (1. 400016) (2. 402168) 摘 要: 利用随机扩增多态性技术(RAPD)研究生姜连作地、施加有机肥后的生姜连作地及新开地 中土壤微生物多样性与生姜青枯病的关系, 结果发现, 连作地的青枯病发病率为 100%, 新开地没 有发病, 而施加有机肥的连作地生姜青枯病发病率降至 37%。发现施加有机肥连作地的真菌、放 线菌数量增加, 平板上不同菌落形态的真菌、细菌、放线菌增加明显。多样性指数分析发现Shannon 指数、Simpson 指数等均显著增加。随机扩增条带出现连作地的条带丰富度较少、新开地的条带 最多、施加有机肥连作地的 RAPD 条带明显多于连作地, 接近新开地, 并出现了一些连作地没有 的特异条带。结果表明, 施加有机肥可以改善土壤微生物群落结构。 关键词: 生姜青枯病, RAPD, 微生物多样性 Controlling Ginger Bacterial Wilt of Soil by Using RAPD Analysis LU He1* ZHANG Bi-Bo2 (1. Department of Microbiology, Chongqing Medical University, Chongqing 400016, China) (2. College of Life Sciences, Chongqing University of Arts and Sciences, Chongqing 402168, China) Abstract: Relationship between bacterial wilt of ginger with its soil biodiversity of freshly plowed soil, con- tinuous cropping soil of ginger and continuous cropping soil receiving ecologically organic fertilizer (EOF) was studied by Random Amplification polymorphic DNA (RAPD) method. The results showed that ginger wilt did not occur in freshly plowed soil, while the ratio of ginger wilt was 100% in continuous cropping soil, and reduced to 37% when the continuous cropping soil receiving ecologically organic fertilizer. The soil mi- crobial number of Fung