六神丸抗恶性肿瘤术后转移的临床研究和对整合素β1、上皮钙黏素影响的实验研究.pdfVIP

提 要 目的：本课题通过运用临床研究与实验研究相结合的方法，观察六神丸治疗恶性 肿瘤术后患者的疗效及其对高转移人肺腺癌 A549 细胞整合素β1（Integrinβ1）和 上皮钙黏素（E-cadherin，简称E-cd）表达的影响。方法：临床研究部分纳入45例 恶性肿瘤术后患者随机分为观察组（化疗/OLF 或 GP 方案+六神丸组）和对照组（化 疗组/OLF 或 GP 方案），其中观察组 24 例，对照组 21 例，均治疗 4 个疗程后比较两 组一、二年内转移率及生存率，治疗前后卡式评分、体重、免疫功能的变化和毒副反 应的出现情况；实验研究部分采用血清药理学方法，通过运用MTT法测定六神丸含药 血清对 A549 细胞的细胞毒作用及生长抑制作用，免疫组化法测定六神丸含药血清对 Integrinβ1 和 E-cd 表达的影响，以对六神丸抗转移的作用机制做进一步的探讨。 结果：临床研究证实，观察组可降低恶性肿瘤术后患者的转移率，提高患者的生存率 和生活质量，稳定并增加体重，提高机体免疫功能，有效减少化疗的毒副反应；实验 研究证实，六神丸含药血清对 A549 细胞有明显抑制作用，六神丸可能通过下调 Integrinβ1和E-cd的表达发挥抗转移作用，与顺铂合用有协同作用。结论:六神丸 联合化疗治疗恶性肿瘤疗效明显，具有增效减毒作用，可显著降低恶性肿瘤术后患者 的转移率，提高患者的生存率。 关键词 六神丸；整合素β1；上皮钙黏素；免疫组化 Clinical Research of Liu-shen-wan Against Cancer Metastasis and Experimental Research of the influence to Integrin beta 1 and Epithelial cadherins Speciality: Internal Medicine of Integrated TCM Author: Hao Guiying Tutor: Professor Li Xiurong Abstract Objective:This topic using the method of the combination of clinical and experimental research，to observe the effect of liu-shen-wan in treating the post-operative patients, and the expressive of integrin beta 1 and epithelial cadherins about high transfer people with lung adenocarcinoma A549 cells . Methods: Clinical research part into 45 patients with cancer patients were randomly divided into trial group (chemotherapy/EP or NP plan + liu-shen-wan) and the control group (chemotherapy group/EP or NP scheme), the observation group 24 cases and control 21 cases.The patients in the two groups were treated 4-treatment. After the treatment, compared the two groups with the recent ratings, KaShi objective curative effect, weight, immune function changes and the reaction and toxicity of the appearance. In th