褐虾过敏原cra c 8蛋白的结构分析和同源建模.pdf

※生物工程 食品科学 2012, Vol. 33, No. 09 177 Cra c 8 陆宗超，王邦平，李振兴 * ( 中国海洋大学食品安全实验室，山东 青岛 266003) 为了进一步认识褐虾中的一种过敏原——磷酸丙糖异构酶过敏原(Cra c 8) 的结构与功能关系，理解食物过 敏原之间产生交叉反应的分子基础，采用生物信息学方法分析 Cra c 8 蛋白的理化性质和各级结构，探讨其与食物 中该蛋白质在分子水平上的异同。结果表明：褐虾 Cra c 8 蛋白是由249 个氨基酸组成的酸性蛋白质，与螯虾、对 虾、三文鱼、小麦、蟑螂中的磷酸丙糖异构酶蛋白具有较高的同源性，亲疏水性区域相似；二级结构预测结果 显示其以 α- 螺旋和不规则折叠为主，均没有 β- 折叠及 β- 转角区域；利用同源建模的方式成功的构建褐虾 Cra c 8 蛋白的空间结构。 生物信息学；磷酸丙糖异构酶；过敏原；同源建模 Structure Analysis and Homology Modeling of Cra c 8 Protein from Crangon crangon LU Zong-chao ，WANG Bang-ping ，LI Zhen-xing* (Food Safety Laboratory, Ocean University of China, Qingdao 266003, China) Abstract ：To further understand the structure-function relationship of the allergen Cra c 8 of triosephosphate isomerase (TPI) from brown shrimps (Crangon crangon) and the molecular basis of cross reaction among food allergens, physical and chemical properties as well as the difference and similarity between Cra c 8 and other triosephosphate isomerases at molecular level were analyzed by a series of bioinformatics softwares. The results showed that Cra c 8 protein was an acidic protein composed of 249 amino acids. The homology of similar proteins was relatively high and hydrophobic and hydrophilic areas had great similarity in Archaeopotamobius sibiriensis , Fenneropenaeus chinensis, Salmo salar, Triticum aestivum and Blattella germanica . The prediction results of secondary structure showed that α-helix and irregular folds were the major structures of Cra c 8 protein, while β-sheet and β-corner regions were absent. The spatial structure of Cra c 8 protein was successfully built by homology modeling. This study will provide a theoretical reference for exploring the activity a