ⅰ期非小细胞肺癌中侵袭与转移相关蛋白的表达及其预后意义.pdf

Ⅰ期非小细胞肺癌中侵袭与转移相关蛋 白的表达及其预后意义 作者：谢泽明，朱志华，张兰军，戎铁华，吴秋良 作者单位：中山大学肿瘤防治中心： 1 胸科，2病理科，广东 广州 510060 【摘要】 目的： 研究Ⅰ期非小细胞肺癌（NSCLC）中侵袭与转移相关蛋白 Cd44v6,癌胚抗原（CEA）, 钙粘连蛋白（E cadherin）,基质金属蛋白酶 2 (MMP  2),MMP 9,β连环素（β Catenin）的表达及其与预后的关系. 方法： 用组 织芯片结合免疫组织化学法检测80例Ⅰ期非小细胞肺癌患者石蜡标本中 Cd44v6，CEA，E cadherin，MMP 2，MMP 9，β Catenin的表达，对表达强 度进行6级评分，结合临床病理数据分析各标志 物的表达与预后的关系. 结果： 本组80例Ⅰ期NSCLC的5 a生存率为68%. 单因素分析中，仅MMP 2有预后意 义（Log rank test,P=0.046）. 在包括Cd44v6，CEA，E cadherin，MMP 2， MMP 9，β Catenin，年龄，性别，病理类型的多因素分析中，也仅有 MMP 2 是影响预后的独立因子，MMP 2高表达者预后较差（P=0.003）. 结论： 在6 种侵袭与转移相关蛋白中，只有MMP 2可作为Ⅰ期NSCLC的预后因子，MMP 2 高表达者预后较差. 【关键词】 癌,非小细胞肺；预后；组织芯片, 生物学标记 Expression of proteins related to invasion and metastasis in stage Ⅰ NSCLC and their prognostic impact XIE Ze Ming, ZHU Zhi Hua, ZHANG Lan Jun, RONG Tie Hua, WU Qiu  Liang Department of Thoracic Surgery, Department of Pathology, Cancer Center, Sun Yat sen University, Guangzhou 510060,China 【Abstract】 AIM: To investigate the expressions of Cd44v6, carcinoembryonic antigen (CEA), E cadherin, matrix metalloproteinase  2(MMP 2), MMP 9, β Catenin in stage Ⅰ non small cell lung cancer (NSCLC) and their prognostic values. METHODS: The expressions of the six proteins were evaluated by immunohistochemistry with tissue chip in paraffin embedded specimens from 80 consecutive NSCLC patients. Clinicopathological data were employed to investigate the expression rules and their prognostic values. RESULTS: The 5 year survival rate of these 80 stage Ⅰ NSCLC patients was 68%. Among the 6 proteins, only MMP2 was of prognostic value in Log rank test（P=0.046）. In Cox multivariate analysis which employed the six biomarkers, including Cd44v6, CEA, E cadherin, MMP 2, MMP 9, β  Catenin, age, sex, and pathological sub type, MMP 2 remained the only independent prognostic factor（P=0.003）. CONCLUSION: Among the six proteins related to cancer invasion and metastasis, MMP 2 is the only biomarker which has prognostic value in stage Ⅰ N