HPLC测定不同产地粉萆薢中原薯蓣皂苷薯蓣皂苷的含量.docxVIP

HPLC法测定不同产地粉萆薢中原薯蓣皂苷和薯蓣皂苷含量邵岩岩，肖潇1，吴玲芳1，朱丹1，杨光辉1，张晓雪1，张兰珍（北京中医药大学中药学院，北京 100102）摘要:目的：建立 HPLC 法测定不同产地粉萆薢中原薯蓣皂苷和薯蓣皂苷含量。方法：采用 Agilent C18 色谱柱（4.6 mm × 150 mm，5 μm），以乙腈 - 水为流动相，梯度洗脱，体积流量为0.8 mL·min-1，检测波长为 208 nm，柱温30 ℃，进样体积20 μL。结果：原薯蓣皂苷在1.73～8.64μg（r = 0. 9996）范围内线性关系良好，平均回收率为101.98 %（RSD = 1.53 %）；薯蓣皂苷在1.03～8.20 μg（r = 0.9991）范围内线性关系良好，平均回收率为101.60 %（RSD = 2.41 %）。10个不同产地粉萆薢中原薯蓣皂苷的含量在0.89 ～2.24 %之间, 薯蓣皂苷的含量在 0.75 ～3.22 %之间。结论：该方法操作简便，定量准确，重现性好，可为粉萆薢药材质量控制提供方法。关键词: 粉萆薢；原薯蓣皂苷；薯蓣皂苷；不同产地；含量测定；高效液相色谱基金资助: 北京中医药大学科研创新团队项目（No.2011-CXTRD-12）Determination of protodioscin and diosgenin inDioscoreahypoglauca from differenthabitats by HPLCSHAO Yan-yan1, XIAO Xiao1,WU Ling-fang1,ZHU Dan1,YANG Guang-hui1, ZHANG Xiao-xue1, ZHANG Lan-zhen1(1School of Chinese MatericaMedica, Beiing University of Chinese Medicine, Beijing 100102, China)Abstract: Objective:To establishan HPLC method for the content determination ofprotodioscin and diosgenin in Dioscoreahypoglaucaand compare the contents from differenthabitats.Methods: The chromatographic separation was performed onan Agilent C18 column (4.6 mm × 150 mm, 5 μm);with mobile phase of acetonitrile –water solution with gradient elution at the flow rate of 0.8mL·min-1; the detection wavelength was 208 nm, thecolumn temperature was 30℃, and the injection volume was 20μL. Results: Protodioscinshowed a goodlinear relationship with the range of 1.73～8.64 μg(r= 0.9996), with the average recovery of101.98 % (RSD= 1.53 %);Diosgeninshowed a goodlinear relationship with the rangeof1.03～8.20μg(r = 0. 9991),withthe averagerecoveryof 101.60 % (RSD=2.41 %).The contents of protodioscin anddiosgeninin D. hypoglauca from 10different habitats werein the range of 0.89～2.24 % and 0.75～3.22 %. Conclusion: The method is fast，simple，accurate and reliable，and can be used to determine the content of protodioscinanddiosgenin in D. hypoglauac. Key words: Dioscoreahypoglauca; protodioscin; diosgenin;different habitats;content determination; HPLCFund assistance: Scie