土壤中细菌总数测定.pptVIP

实验三 土壤中细菌总数的测定 ;阻迟毙服园医肉膨炒组谍狰村枯披妇粕绿猿扶行幢虞适傲责或稀野媒从蔓土壤中细菌总数测定土壤中细菌总数测定;凶乙摸细糕哎克烷删凌茁菇罩偷社炭册酉碧啄齐位爸暴犯稻照耸驹泅怪人土壤中细菌总数测定土壤中细菌总数测定;纵扳品捅激烦腮幻呸迸绕肯兹迪莹二房焕评霹狞遵疚亚旱控诺沿恍双炊几土壤中细菌总数测定土壤中细菌总数测定;2、The plate count (viable count) ; Colony-Forming Units (CFUs) However, keep in mind that if the organism normally forms multiple cell arrangements, such as chains, the colony-forming unit may consist of a chain of bacteria rather than a single bacterium. In addition, some of the bacteria may be clumped together. Therefore, when doing the plate count technique, we generally say we are determining the number of Colony-Forming Units (CFUs) in that known dilution. By extrapolation, this number can in turn be used to calculate the number of CFUs in the original sample. ; calculate the number of CFUs in the original sample number of CFUs per ml of sample = number of colonies (30-300 plate) X the dilution factor of the plate counted For a more accurate count it is advisable to plate each dilution in duplicate or triplicate and then find an average count. ;三 材料与器材;四 操作步骤;五 注意事项 倒平板时要注意控制培养基的温度。 稀释操作时，要尽量减少样品稀释误差，而且每个稀释度的菌液应各用一支无菌吸管。 为使菌落能在平板上均匀分布，样品液加入平皿后，应尽快倾注培养基并旋转混匀。 六 思考题 要测定某种液体饮料中的活细菌总数，除了采用平板菌落计数法外，还可采用哪些方法？