gnrh 及其受体在2 种真骨鱼消化系统中存在的免疫细胞化学证据an .pdf

283 Vol. 28, No.3 2004 6 JOURNAL OF FISHERIES OF CHINA June, 2004 : 1000- 0615(2004) 03- 0261- 06 GnRH 2 1 2 方之平, 潘黔生 ( 1. , 430070; 2. , 430070) :- ( strept avidin biotin-peroxidase complex, SABC) , ( gonadotropin-releasing hormone, GnRH) , ( gonadotropin- releasing hormone receptor,GnRHR)2 (Pelteobagrusf ulvidraco)(Silurus asotus) : GnRH GnRHR ; GnRH GnRHR , ,GnRH GnRHR GnRH GnRH , GnRH :; ;; ; :Q575. 12; S917 :A An immunocytochemical evidence for the presence of GnRH and GnRHR in the digestive system of two teleosts 1 2 FANG Zh-i ping , PAN Qian-sheng (1. College of Animal Science and Technology, Huazhong Agricultural University , uhan 430070, China; 2. College of Fisheries, Huazhong Agricultural University, uhan 430070, China) Abstract: By using the strept avidin biotin-peroxidase complex( SABC) immunocytochemical staining technique, the immunoreactive endocrine cells in the esophagus, cardia, fundus ventriculi, pylorus, foregut, midgut, hindgut, pancreas and liver of oriental sheatfish(Silurus asotus) and yello catfish( Pelteobagrusf ulvidraco) ere localized ith 2 kinds of antisera-gonadotropin releasing hormone(GnRH) and gonadotropin releasing hormone receptor(GnRHR) . The result sho ed that there as GnRH and GnRHR immunoreactive positive reaction in the esophagus, stomach, intestinum, membrana propria, nerve plexus in myenteron, pancreas and pancreatic island of S. asotus and in the digestive sy