尿液试纸的反应原理与伪阴阳性.PDF

福療 尿度 PH Bromothymol Blue 6.3 ug Methyl Red 0.5 ug Phenolphtalein 3.1 ug 理 利 Methyl Red(PH4.6 - 6.2), Bromothymol Blue(PH 6.2 - 7.8) 狀 → 綠 →藍 度 Felton(1921) 1960 尿 臨 : 參 福療 尿 (NITRITE) Hydroxy-Tetrahydro-benzochinolin 15.0 ug Sulfanilamide 12.6 ug 理 尿 (NITRATE)蘭 (NITRITE) Nitrite Aromatic Amine Sufanilamide Acid form Diazonium Salt 3-Hydroxy-1.2.3.4-Tetrahydro-7.8- Benzoquinoline AZO Dye 度 ( 0.03 mg/dl ) 量 (Vit-C)度降 療尿尿 讀 臨 : 參 Nitrite Griess(1879) Weltmann(1922) 來 Fuchs Gutensohn 福療 尿 PROTEIN 3.3.5.5-Tetrachlorophenol- 3.4.5.6-TetrabromoSulfophtalein 2.5 ug Buffer 理 利 Protein error indicator理 TTS PH → 綠 度 (30) (100) (500) mg/dl 1. Quaternary ammonium grop 2. Chlorohexidine 3. Phenazopyridine療 4. Polyvinylpyrrolidone ()….. 臨 參 Protein Error of PH Indicator Sorenson (1909) 福療 尿 GLUCOSE Glucose Oxidase 2.3 U Peroxidase 12.4 U 3.35.5-Tetra-Methyl-Benzindine 46.0 ug GOD 理 Glucose + O ───── D-Gluconolactone + H O 2 2 2 POD H O ─────→ [ O ] + H O 2 2 2 [ O ] + Chromogen (TMB) ────→ TMB (綠 ) 度 (50) (100) (300) (1000) mg/dl 1.