近红外光谱技术快速测定杞菊地黄丸的水分含量-中国现代应用药学.PDF

composition and formulation [J]. Int J Pharm, 2009, 379(1): [J]. Int J Pharm, 2001, 226(1/2): 147-161. 18-24. [8] GUO X, HUANG Z Z, LIU W F, et al. Determination of the [7] FORSTER A, HEMPENSTALL J, TUCKER I, et al. Selection metabolite of anetholtrithion in human plasma by LC-MS/MS of excipients for melt extrusion with two poorly water-soluble [J]. Chin J Pharm(中国医药工业杂志), 2007, 38(6): 444-446. drugs by solubility parameter calculation and thermal analysis 收稿日期：2012-04-22 近红外光谱技术快速测定杞菊地黄丸的水分含量 白雁，刘建营，雷敬卫，谢彩侠，陈志红* ，龚海燕(河南中医学院药学院，郑州 450008) 摘要：目的 应用近红外光谱技术建立一种杞菊地黄丸(浓缩丸) 中水分含量的快速测定方法。方法 以甲苯法测定的样 品中水分的含量为真实值，运用近红外漫反射光谱技术采集96 份杞菊地黄丸(浓缩丸)样品的近红外漫反射光谱，结合偏 最小二乘法(PLS) 建立水分含量的定量分析模型。结果 所建水分校正模型的相关系数(R2) 和内部交叉验证均方差 (RMSECV)分别为 0.988 09 和 0.058 7 ；经外部验证，模型的预测相关系数(r2)和预测均方差(RMSEP)分别为 0.996 9 和 0.075 2 。结论 该方法操作简便，无污染，结果准确可靠，可用于杞菊地黄丸(浓缩丸) 中水分含量的快速测定。 关键词：近红外光谱技术；杞菊地黄丸浓缩丸；水分；快速测定 中图分类号：R917.102 文献标志码：A 文章编号：1007-7693(2013)01-0052-03 Rapid Determination of Moisture in Qiju Dihuang Pills by Near-infrared Spectroscopy Technique * BAI Yan, LIU Jianying, LEI Jingwei, XIE Caixia, CHEN Zhihong , GONG Haiyan(Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China) ABSTRACT: OBJECTIVE To establish a rapid analytical method for moisture in Qiju Dihuang Pills (condensed) by near-infrared diffuse reflectance technique. METHODS First, toluene method was used to determine the content of water in samples. At the same time, NIR diffuse reflectance spectra of 96 samples were collected. Then, Partial Least-Squares (PLS) method was used to create a quantitative model of calibration. RESULTS The correlation coefficients of the PLS