FLT3抑制剂CHMFL-FLT3-122解离常数的测定.PDFVIP

FLT3 抑制剂 CHMFL-FLT3-122 解离常数的测定  * 赵文婷，狄斌 ( 中国药科大学药学院，南京 211198) 摘要：目的 测定CHMFL-FLT3-122 的2 个解离常数。方法 将CHMFL-FLT3-122 溶于一系列的不同pH 的缓冲溶液中， 并在合适的波长处采用UV 分别测定吸光度并计算其pKa1 值；以不同比例的乙腈-水(30 ∶70 、40 ∶60 、50 ∶50 、60 ∶40) 为溶剂配制不同浓度(0.009 ，0.013 ，0.017 mol·L1)的CHMFL-FLT3-122 溶液，用盐酸溶液滴定，利用非对数滴定法(Benet 法)经数学计算推算出其pKa2 值。结果 CHMFL-FLT3-122 存在2 个解离常数，其pKa1 值为3.87±0.14 ，pKa2 值为8.12。 结论 采用UV 和非对数滴定法对CHMFL-FLT3-122 的解离常数进行测定，方法简单快速，准确可靠，可为其制剂的处 方设计、质量标准的研究提供参考。 关键词：CHMFL-FLT3-122；解离常数；紫外分光光度法；非对数滴定法 中图分类号：R917 文献标志码：B 文章编号：1007-7693(2017)07-1002-05 DOI: 10.13748/ki.issn1007-7693.2017.07.015 引用本文：赵文婷, 狄斌. FLT3 抑制剂CHMFL-FLT3-122 解离常数的测定[J].中国现代应用药学, 2017, 34(7): 1002-1006. Determination of Dissociation Constants of FLT3 Inhibitor CHMFL-FLT3-122 * ZHAO Wenting, DI Bin (College of Pharmacy, China Pharmaceutical University, Nanjing 211198, China) ABSTRACT: OBJECTIVE To determine the two dissociation constants of CHMFL-FLT3-122. METHODS Dissolve CHMFL-FLT3-122 in a series of different pH values buffer solutions and the absorbance was measured at appropriate wavelengths by UV to calculate the pKa1. Different proportions of acetonitrile-water mixtures(30 ∶70, 40 ∶60, 50 ∶50, 60 ∶ 40)were used to prepare different concentrations of CHMFL-FLT3-122 solutions(0.009, 0.013, 0.017 mol·L1) which were titrated by hydrochloric acid solution and the pKa2 was calculated by non-logarithmic titration method(Benet method). RESULTS The pKa1 of CHMFL-FLT3-122 was 3.87±0.14 and the pKa2 of CHMFL-FLT3-122 was 8.12. CONCLUSION The determination of the dissociation constants of CHMFL-FLT3-122 by UV and Non-logarithmic titration method is simple, rapid, accurate and reliable. It can be a reference for the prescription design of preparations and the research of quality standard. KEY