镉对小麦叶片DNA 伤害的彗星实验研究 - 环境科学学报.PDF

25 3 Vol.25,No. 3 2005 3 ACTA SCIENTIAE CIRCUMSTANTIAE Mar., 2005 , , .DNA [J].,2005,25(3) :329- 333 LINAijun,ZHANG Xuhong, ZHU ongguan.Thecomet assay detects Cd2induced DNAdamagesin wheat leaves[J].ActaScientiae Circumstantiae,2005,25(3) : 329- 333 DNA ,, * , 100085 - 1 2+ :, , . 01 1mg# L Cd , , 0, 5, 15, 30 min, 100,200,300 mA5, 15, 30min. , , DNADNA , , DNA .,DNA15min, 300mA 15min,., , . :; DNA; :025322468(2005)0320329205 :R99416 :A The comet assay detects Cd2induced DNA damages in wh eat leaves * LIN Aijun, ZHANGXuhong, ZHU ongguan Research Center for Eco2environmental Sciences, ChineseAcademy of Sciences, Beijing 100085 Abstract: The comet assay for the detection of DNA damagesin wheat leaveshad been established, and its application in assessing cadmium (Cd) toxicity was studied. In the experiment, as soon asthe treatment with Cd, thenucleiofwheat leaveswere separatedmechanically and subjectedto comet assay. Theeffectsof denaturation in different time and electrophoresis in various time and current were explored. With the time of denaturation and electrophoresis increased, the sensitivityofthismethod increased simultaneously. But DNA migration also increased in control. Therefore, theappropriate operation conditionshadbeen chosen. The DNA damages (mainly DNA breakages) in nuclei isolated from wheat leaves were detected by the alkaline comet assay. Furthermore, a dose2 dependent increase in DNA damageswasfoundwith increasingthe concentration of Cd in the solution. Keywords:comet assay; DNA damages; wheat leaves , , . Allium [1,3, 4] . (CA) (MCN) Ames , [5]