丙戊酸钠增强阿霉素的体外抗乳腺癌作用 - 南方医科大学学报.pdf

·62 · doi 10.3969/j.issn. 1673-4254.2015.01.12 J South Med Univ, 2015, 35(1): 62-65 基础研究 丙戊酸钠增强阿霉素的体外抗乳腺癌作用丙戊酸钠增强阿霉素的体外抗乳腺癌作用 童旭辉，郑 超，蒋国君，董淑英 蚌埠医学院药学系药理学教研室，安徽 蚌埠 233030 摘要：目的 观察丙戊酸钠对阿霉素体外抗乳腺癌Hs578T 细胞作用的影响。方法 Western blot 检测缝隙连接蛋白Cx43 的表 达；MTT 法检测阿霉素的细胞毒性；Annexin V/PI 双染法观察阿霉素对细胞早期凋亡的影响；Hochest 33258荧光染色法检测阿 霉素对细胞晚期凋亡的影响。结果 Western blot 结果显示丙戊酸钠可以显著增强缝隙连接蛋白Cx43 的表达（P0.01）；MTT 结 果表明在细胞间缝隙连接功能形成的条件下，丙戊酸钠联用阿霉素组的细胞存活率显著低于阿霉素组（P0.01）；Annexin V/PI 双染法显示丙戊酸钠联用阿霉素组的细胞早期凋亡率显著高于阿霉素组（P0.01）；Hochest 33258荧光染色结果显示丙戊酸钠 联用阿霉素组的细胞晚期凋亡率显著高于单用阿霉素组（P0.01）。结论 丙戊酸钠可显著增强阿霉素的细胞毒性及其诱导的 细胞凋亡作用，其机制可能与增强缝隙连接通讯功能有关。 关键词：乳腺癌；丙戊酸钠；阿霉素；凋亡 Sodium valproate enhances doxorubicin cytotoxicity in breast cancer cells in vitro TONG Xuhui, ZHENG Chao, JIANG Guojun, DONG Shuying Department of Pharmacology, Faculty of Pharmacy, Bengbu Medical College, Bengbu 233030, China Abstract: Objective To investigate the effect of sodium valproate, a histone deacetylase inhibitor, on the cytotoxicity of doxorubicin in breast cancer cells. Methods Western blotting was used to assess Cx43 protein expression in breast cancer Hs578T cells exposed to doxorubicin and sodium valproate. MTT assay was used to determine the cytotoxicity of doxorubicin; annexin V/PI double staining and Hochest 33258 fluorescence staining were employed to detect doxorubicin-induced early and late apoptosis, respectively. Results Western blotting showed that sodium valproate significantly increased Cx43 protein expression in Hs578T cells (P0.01). The cells exposed to both sodium valproate and doxorubicin showed significantly lowered cell viability compared with the cells exposed to doxorubicin alone (P0.01). Exposure to both sodium valproate and doxorubicin resulted in significantly increased early and late cell apoptosis rate compared with doxorubicin treatment alone (P0.01). Conclusion sodium va