CXCR4及MMP-13在非小细胞肺癌中表达.doc

CXCR4及MMP-13在非小细胞肺癌中表达[摘要] 目的 检测趋化生长因子受体4（CXCR4）和基质金属蛋白酶-13（MMP-13）在非小细胞肺癌中的表达，探讨二者与非小细胞肺癌临床病理特征的关系，以期为临床工作提供理论支持。 方法 应用免疫组织化学技术检测75例非小细胞肺癌及50例正常肺组织中CXCR4和MMP-13的表达，探讨二者与肿瘤分化程度及淋巴结转移的关系。 结果 非小细胞肺癌中CXCR4和MMP-13表达的阳性率明显高于正常肺组织，CXCR4和MMP-13的表达与非小细胞肺癌的胸膜侵犯、分化程度及有无淋巴结转移密切相关，非小细胞肺癌中CXCR4和MMP-13的表达呈正相关。生存分析显示，CXCR4和MMP-13高表达患者的预后差。 结论 非小细胞肺癌中CXCR4和MMP-13高表达，二者共同促进肿瘤的发生发展，联合检测CXCR4和MMP-13可能与患者的预后有关。 [关键词] 非小细胞肺癌；趋化生长因子受体4；基质金属蛋白酶-13；免疫组织化学 [中图分类号] R734.2 [文献标识码] B [文章编号] 1673-9701（2012）03-0047-02 Expressions and its significance of CXCR4 and MMP-13 in non-small cell lung carcinoma HU Tianjun Department of Thoracic Surgery, Ningbo No.2 Hospital in Zhejiang Province, Ningbo 315010, China [Abstract] Objective To investigate the protein expressions of CXCR4 and MMP-13, observe their relationship and clinical significance on development and metastasis in non-small cell lung carcinoma. Methods The protein expressions of CXCR4 and MMP-13 were detected by immunohistochemistry in the non-small cell lung carcinoma and normal lung tissues. Their expression relationship and different pathological characters were analyzed in non-small cell lung carcinoma. Results The protein expressions of CXCR4 and MMP-13 were higher in the neoplasm than in normal lung tissues. Their expressions were correlated with pleura invasion, differentiation and lymph node metastasis. Positive correlation between CXCR4 and MMP-13 protein was found in neoplasm. Conclusion The higher expressions of CXCR4 and MMP-13 were found in non-small cell lung carcinoma. The synergistic effect of CXCR4 and MMP-13 may promote the development of neoplasm. [Key words] Non-small cell lung carcinoma; CXCR4; Matrix metalloproteinase-13; Immunohistochemistry 浸润和转移是非小细胞肺癌预后不良的重要原因[1]。近来有研究显示非小细胞肺癌的发生发展涉及多种基因和蛋白。趋化生长因子受体4（CXCR4）是基质衍生因子SDF-1的专属受体，可以触发与肿瘤转移有关的多个事件的发生，如肿瘤转移能力、肿瘤细胞的运动能力等[2]。基质金属蛋白酶-13（MMP-13）是基质金属蛋白酶家族的重要成员，对细胞外基质的降解具有重要作用，对促进肿瘤转移有重要价值[3]。本实验应用免疫组织化学技术检测非小细胞肺癌中CXCR4和MMP-13的表达，探讨肿瘤中CXCR4和MMP-13表达的关系