MCM2在非小细胞肺癌表达及意义.doc

MCM2在非小细胞肺癌表达及意义摘要：目的:探讨微小染色体维持蛋白2 (MCM2) 在非小细胞肺癌的表达及其与临床病理特征的关系。方法：采用免疫组化法检测72例非小细胞肺癌组织中MCM2的表达, 分析MCM2的表达与组织学分型、临床分期及分化程度的相关性。结果：MCM2 表达强弱与非小细胞肺癌的组织学分型相关，鳞癌MCM2表达标记指数中位数与腺癌及大细胞癌相比有显著差异（P＜0.01）；MCM2表达强弱与非小细胞肺癌的分化程度密切相关, 分化越低, 表达越明显（P＜0．01）；MCM2 表达强弱与非小细胞肺癌的临床分期无关（P＞0．05）。结论：作为细胞增殖特异性标记物，MCM2的表达与非小细胞肺癌的临床病理特征密切相关。 关键词：微小染色体维持蛋白2；非小细胞肺癌；免疫组化 中图分类号： R734．2 文献标识码： A 文章编号： 1008-2409(2007)06-1207-03 The expression and significance of MCM2 in the non-small cell lung cancer/GONG Li-feng，XIE Guang-shun，RONG Jun∥Shandong Yantai Nurse School， Qingdao University Medical College, Yantai 264000, China Abstract:Objective:To study the expression of minichromosome maintenance protein 2(MCM2) in the non-small cell lung cancer(NSCLC) and the relationship with the clinical features. Methods:The imunohistochemical expression of MCM2 in 72 NSCLC specimensand it’s association with histology, pathologic stage and differentiation grade were analyzed. Results:The expression of MCM2 were associated with histology of NSCLC, the expression of MCM2 in squamous carcinomas was higher than that in large cell carcinoma and adenocarcinoma（P＜0.01）; the expression of MCM2 were associated with differentiation grade of NSCLC,the poorer differentiation,the lower expression（P＜0．01）; the expression of MCM2 weren’t associated with pathologic stage（P＞0．05). Conclusion:As a specific cell proliferation marker, the expression of MCM2 is significantly associated with the clinical features of NSCLC. Key words:minichromosome maintenanceprotein 2; non-small cell lung cancer; immunohistochemistry 恶性肿瘤的一个显著特征是细胞具有自主生长特性,即持续地分裂与繁殖。近年来对细胞生长调控机制的研究,DNA 复制起始调控在细胞生长调控中的核心作用已被提出。一个新的细胞增殖指标MCM 蛋白家族(包括MCM 2～7), 越来越受到人们的关注。它们是细胞周期中启动DNA 复制的关键因子, 已被证明与许多肿瘤的预后密切相关。微小染色体支持蛋白2(minichromosome maintenanceprotein 2,MCM2)是6种微小染色体支持蛋白家族之一,它在哺乳动物的细胞复制中有许可证(licencing factor)作用,它是细胞DNA复制的必要成分,并限制每个细胞周期中DNA只能复制一次, MCM2与细胞复制叉(replicationforks)有关,其功能在复制过程中起DNA解螺旋作用,从而使DNA复制进行[1]。 本研究拟利用免疫组化的方法研究MCM2在肺癌尤其是非小细胞肺癌中的表达，研究其表