的测定结果不准确,而滴定法和比色法均为常量分.PDF

HPLC 快速筛查抗风湿类中成药及保健品中非法添加的 10 种化学药  廖瑜(东莞市食品药品检验所，广东 东莞 523109) 摘要：目的 建立中成药、保健食品中非法添加的10 种抗风湿类化学成分的快速测定方法。方法 采用高效液相色谱法 进行初步筛查。色谱柱：Diamonsil C18(4.6 mm×250 mm ，5 μm) ，流动相：乙腈-0.02 mol·L1 醋酸铵溶液，梯度洗脱；流 1 速：1.0 mL·min ，检测波长：240 nm 。结果 10 种化学药得到了完全分离，检出限2 ng 。结论 本方法操作简单、快 速，灵敏度高，能满足快速筛查抗风湿类中成药及保健品中非法添加化学药的需要。 关键词：中成药；保健品；高效液相色谱法；快速筛查 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2014)07-0868-04 DOI: 10.13748/ki.issn1007-7693.2014.07.026 作者简介：廖瑜，女，主管药师 Tel: (0769 E-mail: liaoyu0709@ ·868 · Chin J Mod Appl Pharm, 2014 July, Vol.31 No.7 中国现代应用药学2014 年 7 月第 31 卷第 7 期 Screening for 10 Anti-rheumatism Chemical Components in Health Food and Chinese Traditional Medicine by HPLC LIAO Yu(Dongguan Institute For Food and Drug Control, Dongguan 523109, China) ABSTRACT: OBJECTIVE To establish a method for rapid detection of 10 anti-rheumatism chemical components adding illegally in health food and traditional Chinese medicine. METHODS Using HPLC to conduct the preliminary screening. The Diamonsil C18(4.6 mm×250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.02 mol·L1 ammonium acetate with linear gradient elution at a flow rate of 1.0 mL·min1, the detection wavelength was 240 nm. RESULTS These ten components were separated completely, and the LODs were 2 ng. CONCLUSION The method is simple, sensitive and quickly to detect chemical components in health food and Chinese Traditional Medicine. KEY WORDS: traditional Chinese medicines; health food; HPLC; rapid detection 近年来，中成药及保健品中非法添加化学药 10 mg，分别置 50 mL 量瓶中，加入甲醇超声使溶 的行为时有发现。其中，抗风湿类中成药中常见 解，放冷，用甲醇定容至刻度，作为对照品储备 的添加成分有解热镇痛抗炎类药如：对乙酰氨基