检测胰液和粪便中K_ras和p5_省略_变对胰腺癌早期诊断的价值_英文_陆星华.pdfVIP

# 1632 # Chinese Medical Journal 2002 ; 115 (11) : 1632-1636 Detecting K-ras and p53 gene mutation from stool and pancreatic juice for diagnosis of early pancreatic cancer LU Xinghua , XU Tong , QIAN Jiaming , WEN Xiaoheng and WU Dongsheng Keywo rds : pancrea ic cancer # de ec io n of K-ras a nd p53 gene mu a ion # s ool # pancrea ic juice # diagno sis Objective To explore new methodsforthe early diagnosisof pancreatic cancerthroughdetectionof K-ras and p53 mutations in pancreatic juice and stool1 Methods 201patients in PU CHospital from1994- 2000 and 60 controlindividualswere enrolled inthis study1 K-ras point mutationwasdetected by PCR-RFLPwhile p53 mutationwas detected by PCR-SSCP1 Results K-ras mutationwasfound inpancreaticjuice in8718% (36/ 41) of pancreatic cancer patientsand 2315% (4/ 17) of benign pancreatic disease patients1 In 261 stool specimens, amplification found mutationssuccessfully in 235 patients (90%) 1 K-ras mutation was found in stool in 88% (66/75) of pancreaticcancer patients, 5111% (24/ 47) of benign pancreatic disease patients and 1916% (9/46) of normalindividuals1 p53 mutation was found in pancreatic juice in 4714% ( 18/38) of pancreatic cancer patientsand 1215% (2/ 16) of benign pancreatic disease patients1 p53 mutation was found in stool in 3711% (23/62) and 1911% (4/21) of chronic pancreatitis patients1 Conclusion K-ras mutation in pancreatic juice has higher diagnosis sensitivity and specificity, and therefore may be used as a supplement inthe diagnosisof pancreatic cancer1 Detectionof K-ras mutation combined with p53 mutation instool canaid inthe screening of pancreatic cancer1