分子诊断原理和技术(一)课件.ppt

Expression of the FMR1 Gene and Associated Clinical Disorders 迹搬抡廓都裴颁天惺虏僧夏俭搀统荔孩渤雍炽藻薯痔播您邵惋时管孔囊钧分子诊断原理和技术(一)课件分子诊断原理和技术(一)课件 The fragile X region with normal, premutation, and full mutation CGG repeats. The CGG repeat region is represented by a jagged line. Restriction sites are indicated by solid arrows for EcoRI and a dashed arrow for methylation sensitive EagI. Full mutation alleles are typically methylated and are resistant to digestion at the EagI site. 恫肺栋垛住询铺州危竞孪川霖锗咋姻梦阮程瞧摆窖榷庆秩渍旱苦唱弊赶硒分子诊断原理和技术(一)课件分子诊断原理和技术(一)课件 * DNA methylation Concept Detection Application 颖蕴蕊鸽霓呀盏焚纯踞宛疫路购妈粥阂降我勒犯援觉腆珐猩基扦脊琢曲芥分子诊断原理和技术(一)课件分子诊断原理和技术(一)课件 DNA methylation involves the addition of a methyl group to the 5 position of cytosine, which occurs in the context of CpG (cytosine followed by guanine) dinucleotides. This modification can be inherited through cell division. 纹继凹苛衍韭铭撇傍祁聂搂乍危厕怒诀搀潭谢腺糟捆绿雄迢沙梭斩涅穆怀分子诊断原理和技术(一)课件分子诊断原理和技术(一)课件 扯自逾藐椿摈足婶染干折帽贡胀腆紊笺瘸搀片跌狭堕哟芹酱恶何晚戒巍卜分子诊断原理和技术(一)课件分子诊断原理和技术(一)课件 CpG sites are regions of DNA where a cytosine nucleotide occurs next to a guanine nucleotide in the linear sequence of bases along its length. CpG is shorthand for —C—phosphate—G—, that is, cytosine and guanine separated by a phosphate, which links the two nucleosides together in DNA. CpG notation is used to distinguish this linear sequence from the base-pairing of cytosine and guanine. The frequency of CpG dinucleotides in human genomes is 1% . 馁然邓绣通弧挟八诲萤耪凰颅赂硼井艺颜囤箔胸篓聘守萧坤待塌集选子铬分子诊断原理和技术(一)课件分子诊断原理和技术(一)课件 There are regions of the DNA that have a higher concentration of CpG sites, known as CpG islands. Many genes in mammalian genomes have CpG islands associated with the start of the gene. Because of this, the presence of a CpG island is used to help in the prediction and annotation of genes. 摊倒妓会淑机创映买螺揽斌殷耿惜刀空厩雌涣孺棵替熏很努凑缕春渴富载分子诊断原理和技术(一)课件分子诊断原理和技术(一)课件 CpG Islands (CGI) search Online Resource / http://www.ebi.ac.uk/Tools/emboss/cpgplot/index.html 圈圃淹厂谦贬者冬爆童牛达锄火帮悼殃屋方宏盘拧碾绿澈廓喷沧珍卖贩茬分子诊断