RHO GTP酶在Ω-3多不饱和脂肪酸抑制人前列腺癌PC-3细胞转移中的作用.pdf

维普资讯 《癌症》ChineseJournalofCancer，2007，26(12)：1281—1286 1281 · 快速报道 · RhoGTP酶在 c^)一3多不饱和脂肪酸抑制 人前列腺癌 PC．3细胞转移中的作用 易 龙 ， 张乾勇， 糜漫天 RoleofRhoGTPaseinInhibitingMetastaticAbilityofHumanProstate CancerCellLinePC-3byc^)-3PolyunsaturatedFattyAcid YiLong，ZHANG Qian—Yong，MIMan—Tian [ABSTRACT]BACKGROUNDOBJECTIVE：Recently，researchesreferto theinfluenceofpolyunsaturatedfattyacid (PUFA) oncancerinitiationand progression had been highly concerned． This studywasto investigate the effects of2 kinds of60—3 PUFA， eicOsapentaenOic acid (EPA) and dOcOsahexaenOicacid (DHA)． onthemetastaticabilityofhumanprostate cancercelllinePC一3．andexploretheroleofRhoGTPase ininhibitingcancer metastasisby60—3PUFA．METHODS：M-I-1-assaywasusedtodeterminethe effects of60—3 PUFA onthe proliferation ofPC一3 cells． Adhesion assay， invasionassay。 andmigrationassaywereused toobservetheeffectsof60—3 PUFA on the metastatic ability ofPC一3 cells． Western blotwas used to 第三军医大学 observetheeffectsof60—3PUFA on the expressionofRhoA， Rac1， Rac2， 军事预防医学院 andCdc42proteinsinPC一3cells． Laserconfocalmicroscopywasusedto 营养与食品卫生学教研室， investigatetheeffectof60—3PUFA onthereorganizationofthemicrofilaments 重庆市营养与食品安全 and microtubules marked bv immunofluorescent cytochemistry technology． 重 点实验室 ．