一种心肌梗死小鼠模型的制备方法.pdfVIP

第26卷 第1期 中 华 中 医 药 学 刊 Vo1．26 NO．1 2 0 0 8年 1月 cHINESE ARCHIVES OF TRADITIONAL CHINESE MEDIC：INE Jan．2 0 0 8 一 种心肌梗死小鼠模型的制备方法 丁红平 ，徐伟建 ，张 君 ，吴艳霞 (1．湖北中医学院硕士研究生，湖北武汉430061；2．湖北中医学院附属医院，湖北武汉430061； 3．华中科技大学同济医学院附属中西医结合医院，湖北武汉430022) 摘 要：目的：制备心肌梗死小鼠动物模型。方法：清洁级昆明小鼠20％乌拉坦麻醉，经口气管插管，于左侧 胸廓第4肋间开胸，结扎左冠状动脉前降支，观察存活情况、心功能、心肌组织病理变化及心肌梗死面积。结果： 完成6o例动物模型制作，2周后存活43只；术后2周心功能明显下降(P0．05)；病理切片可见心肌纤维断裂 溶解，心肌细胞坏死，有炎性细胞浸润；心肌梗死面积为23．6％～31．2％，平均(27．4±1．3)％。结论：该方法操 作简单、创伤小、成功率高、结果可靠。 关键词：心肌梗死 、鼠；模型 中图分类号：R一332 文献标识码：A 文章编号：1673—7717(2008)01—0156—02 One Method of Structure of Myocardial Infarction Model in Mice DING Hong．ping ，XU Wei-jian ，ZHANG Jun ，WU Yan．xia (1．Hubei College of Traditional Chinese Medicine，Wuhan 430061，Hubei，China；2．Affiliated Hospital of Hubei College ofTraditional Chinese Medicine，Wuhan 430061，Hubei，China；3．Affiliated Hospital of Integrated Traditional Chinese and Western Medicine，Tongji Medical College，Huazhong UrIiversity of Science and Technology，Wuhan 430022，Hubei，China) Abstract：Objective：T0 found one model of myocardial infarction in mice．Methods：The mice were anaesthetized by 20％ urethane，then intubated through oral tracheal and performed left anterior thoracotomy between the region of fourth and fiveth intercostals，finally ligated the left anterior descending comnary artery(LAD)．The survivial rate，hean func- tion．pathologic section and the area．of infaretion were observed．Results：60 animals LAD were stitched ，43 8UⅣived ter two weeks．Heart function decreased(P0．05)．The area．ofinfarction were between 23．6％ and 31．2％，its aver． age Was(27．4土1．