pcDNA6 V5-His B哺乳动物表达载体说明.pdf

pcDNA6/V5-His B 编号 载体名称 北京华越洋生物VECT6072 pcDNA6/V5-His B pcDNA6/V5-HisB 载体基本信息 载体名称: pcDNA6/V5-His B 质粒类型: 哺乳动物表达载体；cDNA 表达载体 高拷贝/低拷贝: 高拷贝 克隆方法: 限制性内切酶，多克隆位点 启动子: CMV 载体大小: 5148 bp 5 测序引物及序列: T7 Forward: 5’-TAATACGACTCACTATAGGG-3’ 3 测序引物及序列: BGH Reverse: 5’-TAGAAGGCACAGTCGAGG-3’ 载体标签: V5 Epitope(C-端）；His tag(C-端） 载体抗性: 氨苄青霉素 筛选标记: Blasticidin 克隆菌株: TOP10F´, JM109 and INVaF´ 宿主细胞（系）: 常规细胞系，如293、Hela 等 pcDNA6/V5-His B 载体是cDNA 的表达与克隆载体； CMV 启动子驱动目的基因的过表达； 备注: 易于克隆到其它载体中去； pcDNA6/V5-His A,B,C 仅阅读框不同。 稳定性: 瞬表达 或 稳表达 组成型/诱导型: 组成型 病毒/非病毒: 非病毒 pcDNA6/V5-HisB 载体质粒图谱和多克隆位点信息 pcDNA6/V5-HisB 载体简介 pcDNA6/V5-His A, B, and C are 5.1 kb vectors derived from pcDNA3.1(+) and designed for high-level expression, selection on blasticidin, purification, and detection of recombinant proteins in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. pcDNA6/V5-His A, B, and C 含有以下元件: • Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells • Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide • Blasticidin resistance gene (BSD) from Aspergillus terreus for selection in prokaryotic and eukaryotic cells (Kimura et al., 1994) • Episomal replication in cell lines that are latently infec