一串红叶斑病的病原菌鉴定.PDFVIP

Mycosystema 菌 物 学 报 15 September 2008, 27(5): 634-640 jwxt@ ISSN1672-6472 CN11-5180Q ©2008 Institute of Microbiology, CAS, all rights reserved. 一串红叶斑病的病原菌鉴定 * 张丽丽 张敬泽 胡东维 徐同 浙江大学生物技术研究所 杭州 310029 摘 要：一串红Salvia splendens 叶斑病被发现在杭州市农科所栽培花卉的遮阳网大棚中，是由棒孢霉属 真菌侵染引起的。在田间，病原菌侵染植株的茎和叶片，引起当年移植苗发生茎腐、落叶、植株枯萎和 死亡。通过对病原菌形态学观察以及核糖体DNA ITS 序列分析，证明侵染一串红的病原菌为决明生棒孢 霉菌Corynespora cassiicola。致病性试验结果表明，一串红是决明生棒孢霉菌的寄主。种子带菌检验结果 显示，种子带菌率仅为1%。 关键词：形态学，核糖体DNA ，鉴定，致病性试验，种子带菌检验 Identification of Corynespora leaf spot pathogen of Salvia splendens * ZHANG Li-Li ZHANG Jing-Ze HU Dong-Wei XU Tong Biotechnology Institute, Zhejiang University, Hangzhou 310029, China Abstract ：The Corynespora leaf spot of Salvia splendens was found in the screenhouses covered with black plastic shade nettings for cultivating ornamental plants in the Institute of Hangzhou Agricultural Sciences. The fungal pathogen infected stems and leaves of the host plants and caused stem rot and leaf spots, resulting in earlier defoliation and plant wilting or even death. The morphological observation and rDNA ITS sequence analyses proved that the isolates of the causal agent were C. cassiicola. The pathogenicity tests confirmed that Salvia splendens is a natural host of C. cassiicola. Seed inspection showed that C. cassiicola contaminating rate was only 1%. Key words: morphology, rDNA, pathogenicity test, seed contaminating inspection 基金项目：浙江省自然科学基金（No. Y304071 ）；浙江省教育厅科研项目（No.） * Corresponding author. Tel: 0571 E-mail: jzzhang@ 收稿日期：2007-05-10 ，接受日期：2008-04-11 Vol.27 No.5 635 一串红Salvia splendens Ker Gawl. ，又名西洋红，原产于南美，目前在世界各地广泛栽 培。该花卉在我国种植普