Table2Celldiameterandcrosssectionareaof-第三军医大学学报.DOC

缺氧预处理对制动大鼠胰岛素敏感性影响的研究 王 玉1伍兵2李华凤3杨沛3 （1成都体育学院运动医学系，四川成都 610041；2四川大学华西医院， 四川成都 610041；3四川大学华西第二医院，四川成都 610041；） 摘要 目的：了解缺氧预处理对肢体制动大鼠胰岛素敏感性的影响。方法：将 60只健康、体重150-200g 的雌性SD大鼠按体重配对原则随机分为对照组（CON）、大鼠右后肢石膏固定组（IB）、缺氧预处理+大鼠右后肢石膏固定（HCP）三组，每组20只。HCP组连续对大鼠进行1周的缺氧预处理。每天处理的方法为：氧浓度10%，每次处理30分钟，间隔5分钟，连续处理5次。缺氧预处理完成后，将IB及HCP组大鼠右后肢石膏固定3周,对照组不做特殊处理。3周后将三组大鼠行高胰岛素-正常葡萄糖钳夹技术,术后分离大鼠右比目鱼肌测量重量、肌细胞直径及肌纤维截面积。结果：制动组稳态葡萄糖输入速率、右侧比目鱼肌湿重、肌细胞直径、肌纤维截面积均明显低于对照组,而缺氧预处理组各项指标均有不同程度的改善。结论：缺氧预处理不仅可改善局部制动导致的大鼠骨骼肌萎缩，同时使机体对胰岛素的敏感性也得到了改善。 关键词：缺氧预处理；胰岛素敏感性；高胰岛素-正糖钳夹；制动；骨骼肌萎缩 The Effect of Hypoxic Preconditioning on Insulin the Change of Sensitivite Induced by immobilization in Rats. WANG Yu Chengdu Institute of Physical Education, Chengdu Sichuan 610041,China Abstract: Objective To observe the effects of Hypoxic preconditioning （HCP）in counteracting the insulin sensitivite induced by immobilization. Method 60 female Sprague-Dawley rats were randomly assigned to 3 groups: control (CON), three-week immobilization(IB), and Hypoxic preconditioning plus immobilization (HCP). The rats of HCP group were hypoxic preconditional for 7days.The hypoxia treatment consisted of 5 cycles of 30 min 10% O2 with a 5 min interval daily. The right legs of rats in IB and HCP groups were immobile for three-week. After 3 weeks, hyperinsulinem ic-euglycem ic clamp in conscious rats based on tail arterial and vein catheterization technique were used to evaluate insulin sensitivite and soleus muscle were dissected and weighted. The diameter and cross section area of muscle cell were measured. Result In IB group wet weights of the soleus , the diameter of muscle cell and the area of its cross section decreased obviously as compared with those rats in CON and HCP group. The glucose infusion rate was significant lower than that of CON and HCP group. Conclusion Our data suggest that immobilization can induce soleus atrophy and decruse insulin sensitivite. HCP can partially prevent the soleus atrophy induced by immobilization and increase