川芎药材活性部位的高效液相色谱指纹图谱定性分析方法王嗣岑.pdfVIP

2 4 3 ( ) V ol. 24 N o. 3 2 0 0 3 6 Jo ur nal of X an J ao to ng U n ver s ty ( M ed cal Sc ences) Jun . 2003 川芎药材活性部位的高效液相色谱 指纹图谱定性分析方法 王嗣岑, 朱丽华, 贺浪冲 ( , 71006 1) : 目的 , 方法 , , O DS , ( 30700 . 5) , 276 nm , 7 结果 , , RSD 1. 68% 1. 04 % ; , , 结论 HPL C : ; ; ; : R2 82 . 5 : A :( 2003) 0 3022 10 3 HPLCfingerprint analysis of the active part of Radix Lig ust icum Chuangx iong Hort Wang S cen , Zhu L hua, H e Langchong ( School of Pharmacy , X an J aotong U n vers ty, X an 710061, Ch na) ABSTRACT: Objective T o establ sh a HPL Cf nger pr nt pat tern ( H PL CFP S) f or the a ct v e par t of R ad x L gu st cum Chua ngx o ng Ho r t . and make a compar at ve study of HPL CF PS o f seven k nds o f Rad x L gu st cum Chua ngx o ng Ho rt . fr o m d f f er ent sources . Methods T he samples w er e extr acte d w th e thano l by superson c techn que fo r 20 m n , a nd ex tra cted w th ethyl aceta te. T he O D S co lumn w a s use d as analyt cal co lumn , the mob le phase cons sted o f me thanolw a teracet c ac d ( 30700 . 5) , and the flow r ate w as 1 mLm n- 1 w th U V dete ct o n at 276 nm . Results T he me thod w as pro ve n to be ple c se w th go od re pr o duc b l ty and the R SD w as 1. 6 8% and 1. 04 % u s ng f erul c ac d as the standard compo nent , respe ct v ely . T he spectr um o f the chr o mato gr am s w as s m lar , but the r at o o f com mon peaks are a of d ff e rent sa mples w as d f f er ent . Conclusion T h s metho d ca n be u sed to d ff ere nt ate conv en ent ly and rap dl