NGS用于指导晚期恶性肿瘤治疗的20162016-6-19分析.pptx

NGS用于指导晚期恶性肿瘤治疗2016 ASCO新进展----精准检测，精准治疗Insight on Novel Mechanisms and Precision Care ;基因检测一览;Presented By Claudine Isaacs at 2016 ASCO Annual Meeting;Sequencing is …;近8年，ASCO年会上与NGS相关的摘要数目投稿;内容： NGS指导下的肿瘤免疫治疗 cfDNA检测与肿瘤治疗 NGS在多种实体肿瘤治疗中的应用;Cancer is a disease of …;Presented By Douglas Johnson at 2016 ASCO Annual Meeting;Introduction;Candidate biomarkers for anti-PD-1/PD-L1;Aims;Methods;Mutation load and response;Mutation load and response;Mutation load and response;Mutation load and survival;Specific mutations;Specific mutations;Mutation load comparisons;Mutation load and survival in unselected patients;TCR clonality;Potential implications;Conclusions;内容： NGS指导下的肿瘤免疫治疗 cfDNA检测与肿瘤治疗 NGS在多种实体肿瘤治疗中的应用;ctDNA PPV vs. tissue biopsy (NSCLC, CRC, Breast, Others);Serial Next Generation Sequencing (NGS) of cell free DNA (cfDNA) reveals clonal evolution of AKT1 E17K mutant tumors: analyses from patients enrolled on a phase I basket study of an AKT inhibitor (AZD5363) ;Background – Targetable Driver Oncogenes;Background- Liquid biopsies;Background- Liquid biopsies;Objectives;Study design;Study Schema ;Sample Collection;Patient demographics;br /AKT1 E17K detected and monitored in cfDNA by ddPCR;AKT1 mutant allele fraction (MAF) in cfDNA highly concordant between ddPCR and NGS;NGS of Pre-treatment cfDNA (n=25) captured tumor heterogeneity;Slide 13;Pre-treatment cfDNA captured tumor heterogeneity;Conclusions;内容： NGS指导下的肿瘤免疫治疗 cfDNA检测与肿瘤治疗 NGS在多种实体肿瘤治疗中的应用;二代基因测序在未治疗、转移性EGFR突变肺癌中发现并发的基因改变;[TITLE];二代基因测序胆管肿瘤揭示肿瘤体系变异和化疗耐药性的联系;[TITLE];对主要的中枢神经系统肿瘤进行二代基因测序，作为个体化治疗肿瘤研究项目的一部分，发现与靶向抑制剂治疗敏感性相关的变异;Slide 1;BRCA1/2阴性的乳腺及卵巢肿瘤家族的二代基因测序;[TITLE];二代基因测序发现年轻口腔肿瘤患者的 易感胚系突变;[TITLE];进展期肿瘤患者血浆游离DNA的超深度二代基因测序;总结：肿瘤动态变异带来巨大的个体化差异;总结：肿瘤精准医疗进入以患者为中心的全程管理的时代;谢谢您的呤听！