清热解毒、化瘀通络方对系膜增生性肾炎大鼠肾组织肿瘤坏死因子α、内皮素1的影响.docVIP

　　清热解毒、化瘀通络方对系膜增生性肾炎大鼠肾组织肿瘤坏死因子α、内皮素1的影响 【摘要】 目的 观察清热解毒、化瘀通络全方及其拆方组对系膜增生性肾炎大鼠肾组织肿瘤坏死因子α(TNFα)、内皮素1(ET1)的影响，以探讨清热解毒、化瘀通络全方及其拆方的作用环节。方法 本实验以雄性SD大鼠为研究对象，以隔日牛血清白蛋白灌胃并尾静脉注射葡萄球菌肠毒素B及皮下分点注射完全氟氏佐剂和不完全氟氏佐剂的方法复制系膜增生性肾炎模型。观察各组实验大鼠肾小球细胞外基质增生情况;免疫组织化学法检测肾组织中TNFα及ET1的表达并进行半定量分析。结果 复制系膜增生性肾炎动物模型，光镜下肾小球轻中度系膜增生。模型组和各观察组系膜增生性肾炎实验大鼠肾组织TNFα，ET1表达均有显著增高(Plt;0.01);在降低肾组织TNFα和ET1表达方面，全方组能显著降低两者的表达水平(Plt;0.01)，两个拆方组各有侧重，清热解毒组偏重于降低肾组织TNFα表达，而化瘀通络组偏重于降低肾组织ET1表达。结论 清热解毒、化瘀通络多法合用在系膜增生性肾小球肾炎的 治疗 中较单一疗法更为有效，可从不同作用环节减轻肾脏病理损伤。 【关键词】 肾小球肾炎/中医药疗法; 系膜增生; 肿瘤坏死因子α; 内皮素1; 大鼠; 动物，实验 　　【Abstract】 Objective Observe the effects on TNFα and ET1 expression in renal tissues of rats esangial proliferative glomerulonephritis(MsPGN) by Chinese herbs oving of blood stasis and channel obstruction(RBSCO,Huayu Tongluo),or each of them,in order to explore mechanism of the Chinese herbs.Methods Male SD rats odels of MsPGN ade by taking orally and timecontrolled vein injecting boving serum albumin(BSA),and vein injecting staphylococcus enterotoxin B(SEB),and spot of division of hypodermic injecting plete Freunds adjuvant and not plete Freunds adjuvant.The proliferation of glomerular mesangial cell(MC) and glomerular extracellular matrix(ECM) icroscope on rat models of MsPGN,munohistochemical method,and quantitative analysis ade.Results In the successful mesangial proliferative nephritis models,glomerular mesangial proliferation ild to moderate,and TNFα and ET1 expression in renal tissues increased significantly(Plt;0.01).The treatment of bination of HD ight have different action mechanism,because HD group phased on reducing TNFα expression(Plt;0.01),phased on reducing ET1 expression(Plt;0.01).Conclusions The treatment of bination of HD ore effective than that of single HD or RBSCO,and it can reduce the renal pathological damage through different treatment mechanisms. 　　【Keyal，experiment 　　系膜增生性肾小球肾炎(mesangial proliferative glomerulonephritis,MsPGN)是国内儿童肾小球疾病最常见的病理类型之一[1]。近年来，从毒邪、瘀血角度论治MsPGN取得一定的进展[