一株抗玉米纹枯病内生细菌的分离鉴定及其抗病促生作用.pdfVIP

微生物学通报 AUG 20, 2008, 35(8): 1240~1245 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 一株抗玉米纹枯病内生细菌的分离鉴定 及其抗病促生作用 1 2 1* 辜运富 张云飞 张小平 (1. 625014) (2. 610041) 摘 要: 玉米纹枯病是玉米主要的真菌病害, 从优良玉米品种川单13 号中分离到一株对玉米纹枯 病菌具有明显抑制作用并能够促进玉米生长的内生细菌。通过形态、生理生化特性分析以及 16S rDNA 序列同源性比较, 鉴定该菌株为枯草芽孢杆菌。盆栽试验表明, 菌株能够抑制玉米纹枯病的 发生, 抑菌率为 43.01%, 促生试验表明, 该菌能够显著促进玉米苗的生长, 促生作用与植物生长 刺激素(IAA)的效果相似, 显示出良好的开发前景。 关键词: 玉米纹枯病, 立枯丝核菌, 内生细菌, 16S rDNA Isolation and Identification of One Anti-Rhizoctonis solani Endophytic Bacteria Strain from Corn and Its Antagonism and Promoting Research 1 2 1* GU Yun-Fu ZHANG Yun-Fei ZHANG Xiao-Ping (1. College of Environmental and Resource Science, Sichuan Agricultural University, Ya’an 625014) (2. Institute of Biogas Science, Ministry of Agriculture , Chengdu 610041) Abstract: The corn sheath blight was the main fungus pathogen of the corn. One endophytic bacteria strain which could significantly inhibit corn sheath blight and promote corn growth was isolated from Chuandan 13 corn seeding. According to morphological, biophysiological and biochemical characteristics and 16S rDNA sequence homology, the strain was identified as Bacillus subtilis, pot experiments indicated the strain could inhibit corn sheath blight from happening, the inhibit rate could reach 43.01%; promoting growth e