一株耐镉细菌的分离及其富集Cd的机理 刘红娟.pdfVIP

3　 2 环境 工程学 报 Vo.l3, No.2 2 0 0 9 2 ChineseJournalofEnvironmentalEngineering Feb.2 0 0 9 Cd ＊ 刘红娟　张　慧　党　志 　易筱筠　杨　琛 (, 510006 ) 　　 RC。 16 rDNA, RC(Bacilluscereus)。 , RC Zn、 Cu, RC 。 、 RC, , 150 mg/L , 。 , 。 RC, RC — OH、— NH、— CO— CO— NH— 。 　 　　　 　 X703.1　　　A　　　1673-9108 (2009)02-0 367 -05 Isolationandbio-accumulationmechanismsofaCd-resistingbacterium LiuHongjuan　 ZhangHu　iDangZh　iYiXiaoyun　 YangChen ( choolofEnvironmentalcienceandEngineering, outhChinaUniversityofTechnology, Guangzhou510006) Abstract　Abacterialstrain, namedasRC, possessingstrongercadmium resistanceandaccumulationabil- itieswasisolatedfrom cadmium-contaminatedsoi.lAccordingtoitsmorphologicalandbiochemicalcharacteristics and16 rDNA sequencesanalysi,s itwasidentifiedasBacilluscereu.sGrowthcurvesofRCwereanalyzedunder cultureliquidofdifferentcadmium concentration.sTheresistanceofRC toZnandCuwasstudied. Theresults show thatitsresistancemechanismstodifferentheavymetalsarediscrepan.tThecadmium precipitationanddis- tributioninsideandoutsidethecellwerestudiedwithscanningelectronmicroscopy(EM)andtransmissione- lectronmicroscopy(TEM).W iththe150 mg/Lcadmium concentration, thereweremuchgranularcadmium precipitateonandinsidethecellwal,landalsoprecipitateadheringtothesurfaceofthemycelium.Henc,e pre- cipitationinsideandoutsidethecellmaybeanmiportantwayofcadmium resistanceandaccumulationofthe bacterium.TheaccumulationofCdinthestrainwasobservedbyinfraredspectroscopy, whichindicatesthepos- siblefunctionalgroupsparticipatedduringtheaccumulationofCdare— OH、— NH、— CO— CO— NH—, etc. Keywords　Bacilluscereu;s cadmium; isolation; accumulation 　　, 96 -1, 30 ℃, [ 8] ,